BETA-淀粉樣蛋白片斷25-35

生物活性體外研究體內(nèi)研究 用途與合成方法 MSDS BETA-淀粉樣蛋白片斷25-35價格(試劑級) 上下游產(chǎn)品信息

中文名稱	BETA-淀粉樣蛋白片斷25-35
中文同義詞	BETA-淀粉樣蛋白片斷25-35;淀粉樣Β-蛋白片段25-35;AMYLOID Β-PROTEIN FRAGMENT 25-35 Β-淀粉狀蛋白25-35;BETA-類淀粉蛋白肽(25-35);Β-淀粉狀蛋白25-35;Β-AMYLOID (25-35);Β-淀粉狀蛋白25-35;Β淀粉樣蛋白25-35
英文名稱	AMYLOID BETA-PROTEIN (HUMAN, 25-35) TRIFLUOROACETATE
英文同義詞	Amyloid beta-peptide(25-35);AMYLOID BETA-PROTEIN (HUMAN, 25-35) TRIFLUOROACETATE;H-GLY-SER-ASN-LYS-GLY-ALA-ILE-ILE-GLY-LEU-MET-OH;AMYLOID BETA-PEPTIDE (25-35) (HUMAN);AMYLOID BETA-PROTEIN FRAGMENT 25-35;AMYLOID BETA-PROTEIN (25-35);AMYLOID-BETA PROTEIN (25-35), HUMAN, MOUSE, RAT;Amyloid beta-Protein (25-35) trifluoroacetate salt
CAS號	131602-53-4
分子式	C45H81N13O14S
分子量	1060.27
EINECS號
相關(guān)類別	細胞生物學;多肽;細胞信號和神經(jīng)生物學;多肽，氨基酸;其他生化試劑;生化試劑-蛋白質(zhì);目錄肽;目錄多肽;proteins;Alzheimers  and  Neurodegenerative  Disease  Research;Amyloid  beta  Protein  Fragments;Amyloid  β  Protein  FragmentsNeuropeptides;Peptide;Neurodegenerative  Disease  Peptides;β  Amyloid  Peptides;Amyloid beta-peptide and related;Alzheimer
Mol文件	131602-53-4.mol
結(jié)構(gòu)式

BETA-淀粉樣蛋白片斷25-35 性質(zhì)

BETA-淀粉樣蛋白片斷25-35 用途與合成方法

生物活性

β-Amyloid (25-35) (Amyloid beta-peptide (25-35)) 是阿爾茨海默氏淀粉樣蛋白β肽的Aβ(25-35) 片段，在培養(yǎng)細胞中顯示出神經(jīng)毒性活性。

體外研究

The amino acid sequence of Aβ(25-35) peptide is NH     ₂    -Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-COOH, where the first Gly represents the amino acid 25 and the last Met represents the amino acid 35. Amyloid beta-peptide(25-35) is also investigated in gel state for the first time. Comparative studies are also carried out using vibrational absorption and ECD. The conformational preference of Aβ(25-35) peptide film is also investigated using vibrational absorption and VCD spectroscopy. Amyloid beta-peptide(25-35) induces apoptotic effects on isolated brain mitochondria and the redox state of methionine-35, plays a key role in the induction of programmed cellular death pathways and toxic events.  
 β-Amyloid Aggregation Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).  
 1. Solid Aβ peptide was dissolved in cold hexafluoro-2-propanol (HFIP). The peptide was incubated at room temperature for at least 1h to establish monomerization and randomization of structure.  
 2. The HFIP was removed by evaporation, and the resulting peptide was stored as a film at -20 or -80 ℃.  
 3. The resulting film was dissolved in anhydrous DMSO at 5 mM and then diluted into the appropriate concentration and buffer (serum- and phenol red-free culture medium) with vortexing.  
 4. Next, the solution was age 48h at 4-8 ℃. The sample was then centrifuged at 14000g for 10 min at 4-8 ℃; the soluble oligomers were in the supernatant. The supernatant was diluted 10-200-fold for experiments.  
 Methods vary depends on the downstream applications.

體內(nèi)研究

Rats are injected with Aβ25–35 peptide intracerebroventricularly and compound Danshen (CDS) are subsequently administered once daily for 23 days. Rats’ behavior is monitored using Morris water maze and passive avoidance. Real time PCR and Western blotting are used in determining amyloid precursor protein (APP), β-site APP cleaved enzyme-1(BACE1), Presenilin-1 (PS1), Insulin-degrading enzyme (IDE) and neprilysin (NEP) in hippocampus. The Alzheimer' s disease (AD) model group present with spatial learning and memory impairments. CDS and donepezil administration significantly ameliorate the Aβ25–35 peptide-induced memory impairment in both Morris water maze (P     [3]    .

參考質(zhì)量標準

外觀:白色粉末
純度(HPLC) ≥98.0%
醋酸根含量5.0%～12.0%
水分含量≤8.0%
肽含量≥80.0%

用途 

Amyloid β的功能區(qū)。兼具有神經(jīng)營養(yǎng)和神經(jīng)毒素效果。用于分子生物學研究。

安全信息

MSDS信息

BETA-淀粉樣蛋白片斷25-35 價格(試劑級)

BETA-淀粉樣蛋白片斷25-35 上下游產(chǎn)品信息