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凝膠數(shù)量 | 1-2塊 | 最高電壓 | 220v |
一小時內完成多達四塊小型膠電泳
還可選配 Mini Trans-Blot? 電泳轉印槽進行 western 轉印
軟橡膠墊片與玻板形成嚴密的密封以確保無泄漏灌膠
帶有簡單凸輪的灌制框**確保在任何平面上實現(xiàn)精確對齊
平行排列的灌膠架**能同時灌制兩塊凝膠,彈簧杠桿設計使得軟橡膠沉淀產(chǎn)生良好的密封性
一天內完成雙向凝膠電泳
聚丙烯酰胺凝膠電泳(PAGE 或 SDS-PAGE)
雙向凝膠電泳
篩選新樣本
評估樣本制備條件
凝膠數(shù) | 1–4 |
預制膠 | Mini-PROTEAN? 預制膠 |
手灌膠 | 使用 Mini-PROTEAN 玻板灌制 |
凝膠尺寸(寬 x 長) | 預制: 8.6 x 6.8;手灌: 8.3 x 7.3 |
玻璃板尺寸 | |
短板 | 10.1 x 7.3 |
玻板 | 10.1 x 8.2 |
2 塊凝膠的緩沖液總體積,ml | 700 ml |
4 塊凝膠的緩沖液總體積,ml | 1,000 ml |
SDS-PAGE 典型運行時間 | 35–45 分鐘(在 200 V 恒定電壓下) |
推薦的電源 | PowerPac? Basic 或 PowerPac HC(大電流) |
尺寸(寬 x 長 x 高),cm | 12 x 16 x 18 |
重量,kg/lb | 1.0/2.2 |
凝膠格式 | 小型 |
孔數(shù)或孔類型 | 孔寬度 | 梳厚度 | ||
0.75 mm | 1.0 mm | 1.5 mm | ||
5 | 12.70 mm | 70 μl | 105 μl | 160 μl |
9 | 5.08 mm | 33 μl | 44 μl | 66 μl |
10 | 5.08 mm | 33 μl | 44 μl | 66 μl |
15 | 3.35 mm | 20 μl | 26 μl | 40 μl |
IPG | 6.20 mm | — | 420 μl | 730 μl |
Prep/2-D | ||||
參考孔 | 3.10 mm | 13 μl | 17 μl | 30 μl |
樣品孔 | 67.44 mm | 310 μl | 400 μl | 680 μl |
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![]() | Sample Loading Guide 1653132 Pkg of 1, 15-well, sample loading guide, blue | ![]() | Sample Loading Guide 1653146 Pkg of 1, 10-well, sample loading guide, yellow |
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