化學性質
屬性敏感性對光線和濕度敏感溶解性Soluble in methanol (20 mg/ml)., Soluble in methanol (20 mg/ml).存貯條件儲存溫度 -20°C描述產(chǎn)品介紹5-DTAF is a fluorescent probe that reactive with proteins. Unlike other reactive fluoresceins, 5-DTAF not only reacts with amino groups, but also reacts with thiol groups and even directly reacts with hydroxy groups such as polysaccharides and other alcohols in aqueous solution at pH above 9.Fluoresence: λex 495 nm, λem 516 nm; λex 492 nm, λem 514 nm in 0.1 M Tris pH 9.0別名5-DTAF;Spiro(isobenzofuran-1(3H);9'-(9H)xanthen)-3-one; 5-(4,6-dichloro-1,3,5-triazin-2-yl)amino)-3';6'-dihydroxy;5-DTAF生化機理5-DTAF與蛋白質的反應活性很高。不同于其它反應熒光素,5-DTAF不僅能與氨基發(fā)生反應,還能與巰基反應甚至可與羥基如多糖及水溶液中pH值大于9的其它醇類直接反應。由于其高活性,5-DTAF也可用于標記碳水化合物。應用5-DTAF is a thiol-reactive label for proteins.-----------------------
熔點>325°C儲存條件2-8°C
危險屬性
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WGK Germany3F10-21