點擊查看說明

• Brand

• BD Pharmingen?

• Application

• Bioimaging, Immunofluorescence, Flow cytometry (Tested During Development)   
  

• Regulatory Status

• RUO

Regulatory Status Legend

• RUO

• For Research Use Only. Not for use in diagnostic or therapeutic procedures.

• RUO (GMP)

• For Research Use Only. Not for use in diagnostic or therapeutic procedures. Although not required, these products are manufactured in accordance with Good Manufacturing Practices.

• GPR

• General Purpose Reagent

• IVD

• For In Vitro Diagnostic Use.

• ASR

• Analyte Specific Reagent. Analytical and performance characteristics are not established.

Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.

Description

In cells undergoing apoptosis, oxidative stress, necrosis, and other cellular processes, the mitochondrial membrane potential (Δψm) can become depolarized. For example, in cells undergoing apoptosis, pro-apoptotic Bcl-2 family proteins cause mitochondrial outer membrane permeabilization (MOMP), resulting in the release of cytochrome C and the subsequent activation of caspase-9 and the apoptotic cascade. MOMP often correlates with the loss of inner mitochondrial membrane potential (Δψm), which can be detected using Δψm-sensitive dyes. These cationic, lipophilic dyes accumulate within the mitochondria of healthy cells, but not within mitochondria that have lost Δψm due to induction of apoptosis or treatment with a mitochondrial uncoupler.

BD Pharmingen? MitoStatus TMRE (Tetramethylrhodamine ethyl ester) is a fluorescent dye that is readily sequestered by active mitochondria, allowing for flow cytometric or imaging analysis to assess for apoptosis or mitochondrial depolarization. Non-apoptotic or cells with polarized mitochondria will fluoresce red while apoptotic or cells with depolarizard mitochondria will have diminished levels of red fluorescence. MitoStatus TMRE (Tetramethylrhodamine ethyl ester) has an excitation maximum of 549 nm, but is well-excited by both the blue (e.g. 488 nM) and yellow-green (e.g. 561 nM) lasers. It has an emission maximum of 574 nm.

Product Notices

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.

3. Accutase is a registered trademark of Innovative Cell Technologies, Inc.

4. Cy is a trademark of GE Healthcare.

5. Before staining with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence.

6. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.