詳細說明
| Replacement Information |
|---|
添加到我的收藏夾
Product has been Added to My Favorites
確定
指定了一個無效數(shù)量。產(chǎn)品數(shù)量已被調(diào)整。
數(shù)量域為空。請輸入一個等于或大于1的數(shù)量，才能向您的購物車添加產(chǎn)品。
| Description | |
|---|---|
| Overview | A complete mammalian proteome extraction kit (C-PEK) designed to extract virtually all proteins from both mammalian tissues and culture cells in a simple two step process within a microcentrifuge tube to minimize protein loss. The extraction results in a total protein sample that is a clarified and non-viscous protein solution showing higher resolution and an increased total number of spots in 2-D gel electrophoresis. Sample size: 1x107 - 1x108 cells or 50-100 mg tissue. |
| Catalogue Number | 539779 |
| Brand Family | Calbiochem? |
| Synonyms | C-PEK Mammalian Kit |
| Materials Required but Not Delivered | ? High purity water, conductivity<5 μS (e.g., Merck) ? Cell culture equipment, media etc. for cell growth (e. g., RPMI, DMEM). ? Micropipettes and tips, 10 μl, 200 μl and 1 ml size (e.g., Eppendorf, Gilson or equivalent) ? Cooled centrifuge and rotor for 50 ml tube size (Eppendorf, Heraeus, Nalgen, etc.) ? Cooled microcentrifuge and rotor up to 25,000 x g for 2 ml tube size (Eppendorf, Heraeus, Nalgen etc.) ? Thermomixer or rolling facility (e.g., Eppendorf) ? Optional: Ultracentrifuge and rotor up to 75,000 x g for 2 ml tube size (e.g. Beckman, Sorvall etc.) ? For tissue extraction: Mortar and pestle made from porslin, small spatula. These instruments must be cooled to -70°C or colder prior to be used in the C-PEK procedure! |
| References | |
|---|---|
| References | Yuan, X., et al. 2002. Electrophoresis 23, 1185. Allen, L. 2000. Functional Genomics Nature 405 819. Herbert, B. 1999. Electrophoresis 20, 660. |
| Product Information | |
|---|---|
| Form | 20 Extractions |
| Kit contains | Wash Buffer, Resuspension Buffer, Extraction Reagent, Reducing Reagent, Benzonase? Nuclease (Cat. No. 71206), Glass Beads, and a user protocol. |
| Applications |
|---|
| Biological Information | |
|---|---|
| Sample Type | Mammalian tissues and cultured cells |
| Physicochemical Information |
|---|
| Dimensions |
|---|
| Materials Information |
|---|
| Toxicological Information |
|---|
| Safety Information according to GHS |
|---|
| Safety Information |
|---|
| Product Usage Statements | |
|---|---|
| Intended use | ProteoExtract? Complete Mammalian Proteome Extraction Kit is designed to extract total proteins from mamalian tissue culture cells or mammalian tissue. |
| Storage and Shipping Information | |
|---|---|
| Ship Code | Ambient Temperature Only |
| Toxicity | Multiple Toxicity Values, refer to MSDS |
| Storage | +2°C to +8°C |
| Storage Conditions | ? Wash Buffer The Wash Buffer is provided ready-to-use. The buffer should be stored at 4°C. ? Resuspension Buffer The Resuspension Buffer is provided ready-to-use. It can be stored at 4°C up to 6 month. For prolonged storage freeze the buffer in convenient aliquots at -20°C. Avoid repeated freezing and thawing! ? Extraction Reagent The Extraction Reagent is supplied as dry powder and can be stored unopened at 4°C. The Extraction Reagent should be reconstituted just prior to use as described below. After reconstitution, it can be stored at 4°C up to 6 month. For prolonged storage freeze the buffers in convenient aliquots at -20°C. Avoid repeated freezing and thawing! ? Reducing Agent The Reducing Agent is supplied as dry powder. It can be stored unopened at 4°C. The Reducing Agent should be reconstituted just prior to use as described below. After reconstitution, aliquot the unused material in 60 μl volumes and freeze at -20°C for further use. Avoid repeated freezing and thawing! ? Benzonase? Benzonase? should be stored at 4°C. |
| Avoid freeze/thaw | Avoid freeze/thaw |
| Do not freeze | Ok to freeze |
| Packaging Information |
|---|
| Transport Information |
|---|
| Supplemental Information | |
|---|---|
| Kit contains | Wash Buffer, Resuspension Buffer, Extraction Reagent, Reducing Reagent, Benzonase? Nuclease (Cat. No. 71206), Glass Beads, and a user protocol. |
| Specifications |
|---|
粵公網(wǎng)安備44196802000105號
400-6226-992