See All Kinase Enzyme Systems 
Recombinant human HIPK1 (156–555) was expressed in Sf9 cells using an N-terminal GST tag. HIPK1  homeodomain-interacting protein kinase 1 is a ser/thr protein kinase  a member of the HIPK family. HIPK 1 is a nuclear kinase that phosphorylates homeodomain transcription factors. HIPK1 phosphorylates DAXX,  this leads to its relocalization  subsequent decrease in transcriptional repression activity (1). HIPK1 also interacts with p53  phosphorylates it on serine residues. HIPK1 expression is elevated in breast cancer cell lines  embryonic fibroblasts from HIPK1-null mice showing more susceptibility to apoptosis induced by DNA damage (2).

ADP-Glo? Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted  ATP, which is a substrate in a reaction catalyzed by Ultra-Glo? Luciferase that produces light. The luminescent signal positively correlates with ADP amount  kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo? Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase  ATPase) using up to 1mM ATP.

Kinase Enzyme System contains:

• Kinase: HIPK1, 10μg (Human, recombinant; amino acids 156–555). MW: ~71kDa.

• Substrate: Native Swine Myelin Basic Protein (MBP).

• Other: Reaction Buffer, DTT.

HIPK1 NCBI Database Entry.

Human Kinase Enzyme Systems  Applications.

特點 - 優(yōu)點

• Profile More Compounds In-House: ADP-Glo? Kinase Assay + Kinase Enzyme System is optimized so that you are up  running in no time.

• Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT  supplemental reagents as needed.

• Obtain Reliable Results: The broad dynamic range, the ease of use  better sensitivity obtained with ADP-Glo? Kinase Assay result in less ambiguous data.

注意事項

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.