質(zhì)量水平

100

生物來源

human dermis (fibroblast)

重編程方法

Sendai virus

描述

age (N/A)

manufacturer/tradename

EBiSC^?

性別

female

生長模式

adherent (pluripotent)

應用

cell culture | stem cell: suitable

相關疾病

none

運輸

dry ice

儲存溫度

?196°C

相關類別

Stem Cells

一般描述

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells.  Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.

The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from  neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

細胞系來源

Depositor
Wellcome Trust Sanger Institute

細胞系描述

Derivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Location of primary tissue procurement: Arm
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown

Characterization
Analysis of Undifferentiated Cells
Marker expression: NANOG (+)POU5F1 (OCT-4)(+)SOX2(+)
PluriTest: Pluripotency score: 20.292
Novelty score: 1.735
Microarray data: https://www.ebi.ac.uk/ega/datasets/EGAD00010000775

Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -

Genotyping
Genotyping
Genome-wide analysis:
Exome sequencing
https://www.ebi.ac.uk/ega/studies/EGAS00001000592

聯(lián)系

Additional Cell Line Info

• EBiSC Access Use Agreement (EAUA)

• Cell Line Information Pack (CLIP)

Note: EAUA and CLIP must be completed before order fulfillment

傳代培養(yǎng)常規(guī)

Surface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
CO₂ concentration: 5%
Culture medium: TeSR^? E8^?

法律信息

E8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation

EBiSC is a trademark of Fraunhofer-Gesellschaft

TeSR is a registered trademark of WiCell Research Institute, Inc.