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Product Brief

• Introduction

  Goat Anti-mouse IgG（H+L） secondary antibody, 5 nm Colloidal Gold Conjugate contains affinity-purified antibodies with well-characterized specificity for Mouse IgG (whole molecule) and are useful in the detection of their specified targets through IHC-P,IHC-F,ICC,EM.

  Overview

  Product Name	5nm Colloidal Gold Labeled Secondary Antibody, Goat Anti-Mouse IgG（H+L）
  SKU/Catalog Number	GA1003
  Description	This antibody is purified from antiserum by immunoaffinity chromatography which removes essentially all goat serum proteins, except the specific antibody for mouse IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity in tissue or cell preparations. The goat anti-mouse IgG is conjugated to 5 nm colloidal gold and generates electron dense particles that are visible using electron microscopy.
  Cite This Product	5nm Colloidal Gold Labeled Secondary Antibody, Goat Anti-Mouse IgG (Boster Biological Technology, Wuhan, CHINA, Catalog # GA1003)
  Host	Goat
  Validated Species	Mouse
  Application	EM, IHC, ICC
  Immunogen	Mouse IgG (whole molecule).
  Conjugate	5 nm Colloidal Gold
  Pack Size	0.5ml/1ml

  Properties

  Clonality	Polyclonal
  Storage	At 4°C for one year.

  Background

  EM-grade colloidal gold conjugated secondary antibodies are useful for the applications of light microscopy and immunoblotting, as well as electron microscopy. Monomeric gold-protein conjugates are separated from aggregates by ultracentrifugation and careful separation by density gradients, resulting in consistent and reproducible gold-IgG conjugates. Boster’s colloidal gold conjugated secondary antibodies are guaranteed to have high affinity and low cross-reactivity.

  
  

Instructions

膠體金稀釋液：PBS 或 TBS 緩沖液，加入試劑級 BSA 至 1%。 使用上述膠體金稀釋液，免疫電鏡按 1：20—50 稀釋，免疫組化按 1：50—100 稀釋。膠體金 稀釋后溶液應于當天使用，未用完的應棄置。