詳細說明
概述
產(chǎn)品名稱Anti-ARHGAP25抗體[EPR13233]
描述
兔單克隆抗體[EPR13233] to ARHGAP25
經(jīng)測試應(yīng)用WB,IP,ICC/IF
種屬反應(yīng)性
與反應(yīng): Human
免疫原
Recombinant fragment within Human ARHGAP25 aa 450-600. The exact sequence is proprietary.
Database link: P42331Run BLAST with
Run BLAST with
陽性對照
Jurkat, Ramos and Daudi cell lysates; Jurkat cells.
常規(guī)說明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb? technology. RabMAb? technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放說明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存儲溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA純度Tissue culture supernatant
克隆單克隆
克隆編號EPR13233
同種型IgG
研究領(lǐng)域
Signal Transduction
Signaling Pathway
G Protein Signaling
Small G Proteins
Regulators
Anti-ARHGAP25 antibody [EPR13233] 圖像
Western blot - Anti-ARHGAP25 [EPR13233] antibody (ab181202)
All lanes : Anti-ARHGAP25 antibody [EPR13233] (ab181202) at 1/10000 dilution
Lane 1 : Daudi cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 73 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ARHGAP25 [EPR13233] antibody (ab181202)
Immunofluorescent analysis of Jurkat cells (-20°C Acetone-fixed) labeling ARHGAP25 with ab181202 at 1/100 dilution followed by Goat anti rabbit IgG (AlexaFluor? 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Western blot - Anti-ARHGAP25 [EPR13233] antibody (ab181202)
Anti-ARHGAP25 antibody [EPR13233] (ab181202) at 1/10000 dilution + Ramos cell lysate at 10 μg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 73 kDa
Immunoprecipitation - Anti-ARHGAP25 [EPR13233] antibody (ab181202)
Western blot analysis of immunoprecipitation pellet from Jurkat cell lysate immunoprecipitated using ab181202 at 1/50 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.