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詳細說明
概述
產(chǎn)品名稱Anti-DDX21抗體[EPR14495]
描述
兔單克隆抗體[EPR14495] to DDX21
經(jīng)測試應(yīng)用ICC/IF,IHC-P,WB
種屬反應(yīng)性
與反應(yīng): Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human DDX21 aa 200-300. The exact sequence is proprietary.
Database link: Q9NR30陽性對照
HepG2, HEK293, Jurkat cell lysates. Human infiltrating duct carcinoma of breast tissue, HeLa cells and HepG2 cells.
常規(guī)說明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb? technology. RabMAb? technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Alternative versions available:
Anti-DDX21 antibody (Alexa Fluor? 647) [EPR14495] (ab203744)
性能
形式Liquid
存放說明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存儲溶液Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS純度Tissue culture supernatant
克隆單克隆
克隆編號EPR14495
同種型IgG
研究領(lǐng)域
Epigenetics and Nuclear Signaling
Transcription
Other factors
Epigenetics and Nuclear Signaling
DNA / RNA
RNA Processing
Other
Anti-DDX21 antibody [EPR14495] 圖像
![Western blot - Anti-DDX21 [EPR14495] antibody (ab182156)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab1821566WB.jpg)
Western blot - Anti-DDX21 [EPR14495] antibody (ab182156)
All lanes : Anti-DDX21 antibody [EPR14495] (ab182156) at 1/5000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HEK293 cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 87 kDa
Observed band size : 87 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX21 [EPR14495] antibody (ab182156)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab182156IHC.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX21 [EPR14495] antibody (ab182156)
Immunohistochemical analysis of paraffin-embedded Human infiltrating duct carcinoma of breast tissue, staining DDX21 with ab182156 at 1/100 dilution. Hematoxylin was used as a counterstain.
![Immunocytochemistry/ Immunofluorescence - Anti-DDX21 [EPR14495] antibody (ab182156)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab182156IF1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DDX21 [EPR14495] antibody (ab182156)
Immunofluorescence analysis of HeLa cells, staining DDX21 (red) with ab182156 at 1/100 dilution. An Alexa Fluor?555 goat anti rabbit IgG was used as a secondary antibody. Cells were fixed with -20℃ Acetone. Nuclei were counterstained with DAPI (blue).
![Immunocytochemistry/ Immunofluorescence - Anti-DDX21 [EPR14495] antibody (ab182156)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab182156IF2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DDX21 [EPR14495] antibody (ab182156)
Immunofluorescence analysis of HepG2 cells, staining DDX21 (green) with ab182156 at 1/100 dilution. An Alexa Fluor?488 goat anti rabbit IgG was used as a secondary antibody. Cells were fixed in 4% paraformaldehyde. Nuclei were counterstained with DAPI (blue).
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