概述

• 產(chǎn)品名稱Anti-DUSP5抗體[EPR19684]
     

• 描述

  兔單克隆抗體[EPR19684] to DUSP5

• 經(jīng)測(cè)試應(yīng)用WB

• 種屬反應(yīng)性

  與反應(yīng): Mouse, Human    
   

• 免疫原

  Recombinant full length protein within Human DUSP5 aa 1 to the C-terminus. The exact sequence is proprietary.
  Database link: Q16690

       Run BLAST with      Run BLAST with    

• 陽(yáng)性對(duì)照    

• WB: Human DUSP5 full length recombinant protein; Mouse DUSP5 full length recombinant protein; MEF (WT) whole cell lysate.

• 常規(guī)說(shuō)明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam's RabMAb^? technology. RabMAb^? technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

性能

• 形式Liquid

• 存放說(shuō)明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存儲(chǔ)溶液Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 40% Glycerol, 0.05% BSA

• 
  

• 純度Protein A purified

• 克隆單克隆

• 克隆編號(hào)EPR19684

• 同種型IgG

• 研究領(lǐng)域

• Signal Transduction

• Protein Phosphorylation

• Tyrosine Phosphatases

Anti-DUSP5 antibody [EPR19684] 圖像

• 

  Western blot - Anti-DUSP5 antibody [EPR19684] (ab200708)          

     All lanes : Anti-DUSP5 antibody [EPR19684] (ab200708) at 1/1000 dilution
  
  Lane 1 : MEF (Mouse embryonic fibroblast cell line) (WT) whole cell lysate
  Lane 2 : MEF (Mouse embryonic fibroblast cell line) (DUSP5 KO) whole cell lysate
  
  Lysates/proteins at 20 μg per lane.
  
  Secondary
  Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
  
  Predicted band size : 42 kDa
  Observed band size : 42 kDa
  
  
  Exposure time : 3 minutes    

  Blocking/Dilution buffer: 5% NFDM/TBST.

  Lysates were kindly provided by Steve Keyes from Dundee University.

   

• 

  Western blot - Anti-DUSP5 antibody [EPR19684] (ab200708)          

     Anti-DUSP5 antibody [EPR19684] (ab200708) at 1/5000 dilution + Human DUSP5 full length recombinant protein at 0.01 μg
  
  Secondary
  Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
  
  Predicted band size : 42 kDa
  
  
  Exposure time : 8 seconds    

  Blocking/Dilution buffer: 5% NFDM/TBST.

  Human DUSP5 full length recombinant protein contain aa1-384 with a His-Tag?. This recombinant human full length protein was made in-house.

   

• 

  Western blot - Anti-DUSP5 antibody [EPR19684] (ab200708)          

     Anti-DUSP5 antibody [EPR19684] (ab200708) at 1/1000 dilution + Mouse DUSP5 full length recombinant protein at 0.01 μg
  
  Secondary
  Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
  
  Predicted band size : 42 kDa
  
  
  Exposure time : 4 seconds    

  Blocking/Dilution buffer: 5% NFDM/TBST.

  Mouse DUSP5 full length recombinant protein contain aa1-384 with a His-Tag?. This recombinant mouse full length protein was made in-house.