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詳細(xì)說(shuō)明
概述
產(chǎn)品名稱Anti-gamma Sarcoglycan抗體[EPR17862-40]
描述
兔單克隆抗體[EPR17862-40] to gamma Sarcoglycan
經(jīng)測(cè)試應(yīng)用IP,IHC-P,WB
種屬反應(yīng)性
與反應(yīng): Human
免疫原
Recombinant fragment within Human gamma Sarcoglycan aa 50 to the C-terminus. The exact sequence is proprietary.
Database link: Q13326Run BLAST with
Run BLAST with 
陽(yáng)性對(duì)照
WB: Human skeletal muscle, MCF-7, Human fetal brain, Human fetal heart, Human fetal spleen or Human fetal kidney whole cell lysate. IHC-P: Human skeletal muscle.
常規(guī)說(shuō)明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb? technology. RabMAb? technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放說(shuō)明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存儲(chǔ)溶液pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA純度Protein A purified
克隆單克隆
克隆編號(hào)EPR17862-40
同種型IgG
研究領(lǐng)域
Signal Transduction
Cytoskeleton / ECM
Extracellular Matrix
ECM Proteins
Other ECM Proteins
Anti-gamma Sarcoglycan antibody [EPR17862-40] 圖像
![Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab203112WB1.jpg)
Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)
Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112) at 1/10000 dilution + Human skeletal muscle lysate at 20 μg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 32 kDa
Observed band size : 32 kDa
Exposure time : 15 secondsBlocking/diluting buffer 5% NFDM/TBST
This protein is expressed in skeletal and heart muscle.
![Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab203112WB2.jpg)
Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)
Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112) at 1/2000 dilution + MCF-7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 μl
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 32 kDa
Observed band size : 32 kDa
Exposure time : 3 minutesBlcking/Dilution buffer 5% NFDM/TBST
This protein is expressed in skeletal and heart muscle.
![Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab203112WB3.jpg)
Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)
All lanes : Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112) at 1/2000 dilution
Lane 1 : Human fetal brain whole cell lysate
Lane 2 : Human fetal heart whole cell lysate
Lane 3 : Human fetal kidney whole cell lysate
Lane 4 : Human fetal spleen whole cell lysate
Lysates/proteins at 10 μg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 32 kDa
Observed band size : 32 kDa
Blocking/DIlution buffer 5% NFDM/TBST.
ab181602 Anti-GAPDH antibody [EPR16891] used as a control.
This protein is expressed in skeletal and heart muscle.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab203112HC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling gamma Sarcoglycan with ab203112 at 1/500 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a 1/500 dilution. Cell membrane and weakly cytoplasmic staining on Human skeletal muscle tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is ab97051 Goat Anti-Rabbit IgG H&L (HRP).
![Immunoprecipitation - Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab203112112.jpg)
Immunoprecipitation - Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)
gamma Sarcoglycan was immunoprecipitated from 1mg of Human fetal kidney whole cell lysate with ab203112 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab203112 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Human fetal kidney whole cell lysate10 μg (Input). Lane 2: ab203112 IP in Human fetal kidney whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203112 in Human fetal kidney whole cell lysate.
Exposure time: 3 secondsBlocking/Dilution buffer 5% NFDM/TBST.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab203112HC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling gamma Sarcoglycan with ab203112 at 1/500 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a 1/500 dilution. Counter stained with Hematoxylin. No staining on Human colon tissue is observed.
Negative control: Used PBS instead of primary antibody, secondary antibody is ab97051 Goat Anti-Rabbit IgG H&L (HRP).
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