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詳細(xì)說(shuō)明
概述
產(chǎn)品名稱Anti-DPP6抗體[EPR15944-24]
描述
兔單克隆抗體[EPR15944-24] to DPP6
經(jīng)測(cè)試應(yīng)用WB,IHC-P
種屬反應(yīng)性
與反應(yīng): Mouse, Rat, Human
免疫原
Recombinant fragment within Human DPP6 aa 400-550. The exact sequence is proprietary.
Database link: P42658Run BLAST with
Run BLAST with 
陽(yáng)性對(duì)照
WB: Rat brain lysate, Human fetal brain lysate, Mouse brain lysate. IHC-P: Human and Mouse cerebral cortex tissues.
常規(guī)說(shuō)明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb? technology. RabMAb? technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放說(shuō)明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存儲(chǔ)溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA純度Protein A purified
克隆單克隆
克隆編號(hào)EPR15944-24
同種型IgG
研究領(lǐng)域
Signal Transduction
Cytoskeleton / ECM
Extracellular Matrix
ECM Enzymes
Other Enzymes
Anti-DPP6 antibody [EPR15944-24] 圖像
![Western blot - Anti-DPP6 antibody [EPR15944-24] (ab198506)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab198506WB1.jpg)
Western blot - Anti-DPP6 antibody [EPR15944-24] (ab198506)
All lanes : Anti-DPP6 antibody [EPR15944-24] (ab198506) at 1/5000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Human fetal brain lysate
Lysates/proteins at 10 μg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 98 kDa
Observed band size : 115 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature PMID: 12575952
![Western blot - Anti-DPP6 antibody [EPR15944-24] (ab198506)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab198506WB2.jpg)
Western blot - Anti-DPP6 antibody [EPR15944-24] (ab198506)
Anti-DPP6 antibody [EPR15944-24] (ab198506) at 1/1000 dilution + Mouse brain lysate at 10 μg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 98 kDa
Observed band size : 115 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature PMID: 12575952.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP6 antibody [EPR15944-24] (ab198506)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab198506HC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP6 antibody [EPR15944-24] (ab198506)
Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling DPP6 with ab198506 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cell membrane and cytoplasm staining of Human cerebral cortex tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP6 antibody [EPR15944-24] (ab198506)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab198506HC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP6 antibody [EPR15944-24] (ab198506)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling DPP6 with ab198506 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. No staining of Human liver tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP6 antibody [EPR15944-24] (ab198506)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab198506HC3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP6 antibody [EPR15944-24] (ab198506)
Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling DPP6 with ab198506 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cell membrane and cytoplasm staining of Mouse cerebral cortex tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP6 antibody [EPR15944-24] (ab198506)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab198506HC4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP6 antibody [EPR15944-24] (ab198506)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling DPP6 with ab198506 at 1/500 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. No staining of Mouse liver tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
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