詳細(xì)說明
概述
產(chǎn)品名稱Anti-C4BPA抗體[EPR14780]
描述
兔單克隆抗體[EPR14780] to C4BPA
經(jīng)測試應(yīng)用WB,IHC-P
種屬反應(yīng)性
與反應(yīng): Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human C4BPA aa 50-150. The exact sequence is proprietary.
Database link: P04003陽性對照
WB: Human plasma lysate. IHC-P: Human transitional cell carcinoma of bladder tissue.
常規(guī)說明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb? technology. RabMAb? technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放說明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存儲溶液pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA純度Protein A purified
克隆單克隆
克隆編號EPR14780
同種型IgG
研究領(lǐng)域
Immunology
Innate Immunity
Complement
Classical Pathway
Anti-C4BPA antibody [EPR14780] 圖像
Western blot - Anti-C4BPA antibody [EPR14780] (ab200345)
Anti-C4BPA antibody [EPR14780] (ab200345) at 1/5000 dilution + human plasma lysate at 20 μg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 67 kDa
Observed band size : 67 kDa
Exposure time : 30 seconds5% NFDM/TBST: Blocking and diluting buffer.
Western blot - Anti-C4BPA antibody [EPR14780] (ab200345)
All lanes : Anti-C4BPA antibody [EPR14780] (ab200345) at 1/5000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human fetalkidney lysate
Lane 3 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lane 4 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 67 kDa
Observed band size : 67 kDa
Exposure time : 30 seconds5% NFDM/TBST: Blocking and diluting buffer.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4BPA antibody [EPR14780] (ab200345)
Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling C4BPA using ab200345 at 1/100 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
Inset image: negative control obtained using PBS instead of ab200345 and secondary antibody only.
Note: Plasma staining on human transitional cell carcinoma of bladder tissue was observed.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4BPA antibody [EPR14780] (ab200345)
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling C4BPA using ab200345 at 1/100 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
Inset image: negative control obtained using PBS instead of ab200345 and secondary antibody only.
Note: No staining on human skeletal muscle tissue was observed.