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詳細(xì)說明
概述
產(chǎn)品名稱Anti-Dkk3抗體[EPR15611]
描述
兔單克隆抗體[EPR15611] to Dkk3
經(jīng)測試應(yīng)用WB,ICC/IF,IP
種屬反應(yīng)性
與反應(yīng): Mouse, Rat, Human
免疫原
Recombinant fragment within Human Dkk3 aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: Q9UBP4Run BLAST with
Run BLAST with 
陽性對照
Human fetal brain, heart and kidney lysates; HepG2 cells.
常規(guī)說明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb? technology. RabMAb? technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Alternative versions available:
Anti-Dkk3 antibody (Alexa Fluor? 488) [EPR15611] (ab201262)
Anti-Dkk3 antibody (HRP) [EPR15611] (ab201748)
性能
形式Liquid
存放說明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存儲溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA純度Protein A purified
克隆單克隆
克隆編號EPR15611
同種型IgG
研究領(lǐng)域
Stem Cells
Signaling Pathways
Wnt
Secreted
Signal Transduction
Adapters
Transmembrane
Anti-Dkk3 antibody [EPR15611] 圖像
![Western blot - Anti-Dkk3 [EPR15611] antibody (ab186409)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab18640909a.jpg)
Western blot - Anti-Dkk3 [EPR15611] antibody (ab186409)
Anti-Dkk3 antibody [EPR15611] (ab186409) at 1/10000 dilution + Human fetal brain lysate at 20 μg
Secondary
Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution
Predicted band size : 38 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
![Western blot - Anti-Dkk3 [EPR15611] antibody (ab186409)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab18640909b.jpg)
Western blot - Anti-Dkk3 [EPR15611] antibody (ab186409)
All lanes : Anti-Dkk3 antibody [EPR15611] (ab186409) at 1/5000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal heart lysate
Lysates/proteins at 10 μg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 38 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
![Immunocytochemistry/ Immunofluorescence - Anti-Dkk3 [EPR15611] antibody (ab186409)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab18640909c.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Dkk3 [EPR15611] antibody (ab186409)
Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling Dkk3 with ab186409 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor? 488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
![Immunoprecipitation - Anti-Dkk3 [EPR15611] antibody (ab186409)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab18640909d.jpg)
Immunoprecipitation - Anti-Dkk3 [EPR15611] antibody (ab186409)
Western blot analysis of Dkk3 in Human fetal brain lysate immunoprecipitated with ab186409 at 1/50 dilution (Lane 1). Lane 2: PBS instead of Human fetal brain lysate.
Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
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