詳細(xì)說明
概述
產(chǎn)品名稱Anti-Carbonic Anhydrase I抗體[EPR5193]
描述
兔單克隆抗體[EPR5193] to Carbonic Anhydrase I
經(jīng)測試應(yīng)用WB,ICC,IHC-Fr
種屬反應(yīng)性
與反應(yīng): Mouse, Rat, Human, Zebrafish
免疫原
A synthetic peptide corresponding to C-terminal residues of Human Carbonic Anhydrase 1
陽性對照
K562 and TF1 cell lysates
常規(guī)說明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb? technology. RabMAb? technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Alternative versions available:
Anti-Carbonic Anhydrase I antibody (HRP) [EPR5193] (ab202929)
性能
形式Liquid
存放說明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
存儲(chǔ)溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
純度Tissue culture supernatant
克隆單克隆
克隆編號EPR5193
同種型IgG
研究領(lǐng)域
Cell Biology
Other Antibodies
Other Antibodies
Cardiovascular
Blood
Other
Anti-Carbonic Anhydrase I antibody [EPR5193] 圖像
Immunohistochemistry (Frozen sections) - Anti-Carbonic Anhydrase I [EPR5193] antibody (ab108367) This image is courtesy of an Abreview submitted by Dr Ryan MacDonald
ab108367 staining Carbonic Anhydrase I in zebrafish retina sections by IHC-Fr. The tissue was fixed with paraformaldehyde and an antigen retrieval step was performed with sodium citrate pH 6. Blocking of the sample was done with 5% BSA in PBS containing 01% Tween 20 and 0.5% Triton X, for 60 minutes at 23°C, followed by staining with ab108367 at 1/200 in blocking solution for 16h at 4°C. An alexa 488 conjugated goat anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei are stained in blue with DAPI. Carbonic Anhydrase I expression can be observed in horizontal cells and Muller cells(in green).
See Abreview
Western blot - Carbonic Anhydrase I antibody [EPR5193] (ab108367)
All lanes : Anti-Carbonic Anhydrase I antibody [EPR5193] (ab108367) at 1/10000 dilution
Lane 1 : K562 cell lysates
Lane 2 : TF1 cell lysates
Lysates/proteins at 10 μg per lane.
Predicted band size : 29 kDa