詳細(xì)說明
概述
產(chǎn)品名稱Anti-NME3抗體[EPR13117]
描述
兔單克隆抗體[EPR13117] to NME3
經(jīng)測試應(yīng)用WB,IHC-P,Flow Cyt,IP
種屬反應(yīng)性
與反應(yīng): Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NME3 aa 50-150. The exact sequence is proprietary.
Database link: Q13232陽性對照
MOLT4, Raji and HeLa cell lysates; Human cardiac muscle and kidney tissues; HeLa and MOLT4 cells.
常規(guī)說明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb? technology. RabMAb? technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放說明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存儲(chǔ)溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA純度Protein A purified
克隆單克隆
克隆編號(hào)EPR13117
同種型IgG
研究領(lǐng)域
Metabolism
Pathways and Processes
Metabolic signaling pathways
Energy transfer pathways
Energy Metabolism
Signal Transduction
Metabolism
Energy Metabolism
Signal Transduction
Second Messenger
Nucleotide Messenger
GTP
Anti-NME3 antibody [EPR13117] 圖像
Immunoprecipitation - Anti-NME3 [EPR13117] antibody (ab181257)
Western blot analysis of immunoprecipitation pellet from MOLT4 cell lysate immunoprecipitated using ab181257 at 1/50 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.Flow Cytometry - Anti-NME3 [EPR13117] antibody (ab181257)
Flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling NME3 with ab181257 at 1/170 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NME3 [EPR13117] antibody (ab181257)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NME3 with ab181257 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NME3 [EPR13117] antibody (ab181257)
Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling NME3 with ab181257 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Western blot - Anti-NME3 [EPR13117] antibody (ab181257)
All lanes : Anti-NME3 antibody [EPR13117] (ab181257) at 1/2000 dilution
Lane 1 : MOLT4 cell lysate
Lane 2 : Raji cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 19 kDa