• Species Reactivity

  Mouse

• Specificity

  Detects mouse TREM-1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross?reactivity with recombinant human (rh) TREM-1 is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse TREM-1    
  Ala21-Ser202    
  Accession # Q9JKE2

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.1 μg/mL

  Recombinant Mouse TREM?1 Fc Chimera (Catalog # )

• 
  

• Flow Cytometry

  0.25 μg/10    ⁶ cells

  Mouse blood GR?1    ⁺ granulocytes

• 
  

• Agonist Activity

  Measured by its ability to stimulate TNF-alpha secretion by P388D1 mouse lymphoma cells (ATCC # TIB-63). Bouchon, A.     et al. (2001) Nature,     410:1103 and Bouchon, A.     et al. (2000) J. Immunology,     164:4991.    
  The ED    ₅₀ for this effect is typically 1?3 μg/mL.

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

• Immunocytochemistry

  5-15 μg/mL

  See below

• 
  

• Mouse TREM-1 Sandwich Immunoassay

  Reagent    

• ELISA Capture (Matched Antibody Pair)

  0.2-0.8 μg/mL 

  Mouse TREM?1 Antibody (Catalog # )

• ELISA Detection (Matched Antibody Pair)

  0.1-0.4 μg/mL 

  Mouse TREM?1 Biotinylated Antibody (Catalog # )

• ELISA Standard

   

  Recombinant Mouse TREM-1 Fc Chimera Protein, CF (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TREM-1

TREM-1 (Triggering Receptor Expressed on Myeloid cells) is a type I transmembrane protein having a single Ig-like domain. It associates with the adapter protein, DAP12, to deliver an activating signal. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1 is expressed on blood neutrophils and a subset of monocytes, and expression is up-regulated by bacterial LPS. Engagement of TREM-1 with a monoclonal antibody leads to expression of IL-8, MCP-1, and TNF-alpha suggesting that this receptor plays an important role in inflammatory responses. TREM-1 is expressed at high levels on neutrophils of patients with microbial sepsis and in mice with LPS-induced shock. Blockade of TREM-1 with a TREM-1/Fc fusion protein protected mice against LPS-induced shock.

• References:

1. Bouchon, A. (2000) J. Immunol. 164:4991.

2. Bouchon, A. (2001) Nature 410:1103.

3. Nathan, C. and A. Ding (2001) Nature Med. 7:530.

• Long Name:

  Triggering Receptor Expressed on Myeloid Cells 1

• Entrez Gene IDs:

  54210 (Human); 58217 (Mouse)

• Alternate Names:

  CD354 antigen; CD354; TREM1; TREM-1; TREM-1Triggering receptor expressed on monocytes 1; triggering receptor expressed on myeloid cells 1; triggering-receptor TREM1