• Species Reactivity

  Human, Mouse, Rat

• Specificity

  Detects human, mouse, and rat XPB in Western blots.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant human XPB    
  Met1-Lys375    
  Accession # P19447

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: XPB

XPB (Xeroderma pigmentosum group B complementing protein; also ERCC3 and TFIIH 89 kDa subunit) is an 86 kDa, nuclear member of the RAD25/XPB subfamily, helicase family of proteins. It is expressed in multiple cell types, including neurons, fibroblasts and keratinocytes. XPB is part of the 10 subunit TFIIH complex. As such, it appears to participate in both nucleotide excision repair (NER) and transcription initiation from RNA polymerase II promoters. During NER, XPB utilizes an intrinsic ATPase activity to resolve nucleotide excision and repair. Human XPB is 782 amino acids (aa) in length. It contains an NLS (aa 6?18), multiple acidic-rich regions, an ATPase region (aa 327?488) and one helicase domain (aa 542?702). Over aa 1?375, human XPB shares 93% aa identity with mouse XPB.

• Long Name:

  Xeroderma Pigmentosum B

• Entrez Gene IDs:

  2071 (Human); 13872 (Mouse); 291703 (Rat)

• Alternate Names:

  Basic transcription factor 2 89 kDa subunit; BTF2 p89; BTF2; DNA excision repair protein ERCC-3; DNA repair protein complementing XP-B cells; EC 3.6.1; EC 3.6.4.12; ERCC3; excision repair cross-complementing rodent repair deficiency, complementationgroup 3 (xeroderma pigmentosum group B complementing); GTF2H; RAD25; TFIIH basal transcription factor complex 89 kDa subunit; TFIIH basal transcription factor complex helicase XPB subunit; TFIIH p89; TFIIH; Xeroderma pigmentosum group B-complementing protein; xeroderma pigmentosum, complementation group B; XPB; XPBC; XPBTFIIH 89 kDa subunit