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Human/Mouse ZNF281 Affinity Purified Polyclonal Ab 25 UG

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Human/Mouse ZNF281 Affinity Purified Polyclonal Ab 25 UG信息二維碼

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產(chǎn)品介紹

    基本參數(shù)

    詳細說明

    • Species Reactivity

      Human, Mouse

    • Specificity

      Detects human and mouse ZNF281 in Western blots.

    • Source

      Polyclonal Sheep IgG

    • Purification

      Antigen Affinity-purified

    • Immunogen

      E. coli-derived recombinant human ZNF281    
      aa 681-895    
      Accession # Q9Y2X9

    • Formulation

      Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

    • Label

      Unconjugated

    Applications

    • Recommended    
      Concentration

      Sample

    • Western Blot

      1 μg/mL

      See below


    Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

    Data Examples

    Western Blot      
         

    Detection of Human and Mouse ZNF281 by Western Blot.

    Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF?7 human breast cancer cell line, and A20 mouse B cell lymphoma cell line. Gels were loaded with 30 μg of whole cell lysate (WCL), XXX μg of cytoplasmic (Cyto), and XXX μg of nuclear extracts (Nuc). PVDF Membrane was probed with 1 μg/mL of Sheep Anti-Human/Mouse ZNF281 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6350) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # ). A specific band was detected for ZNF281 at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using .

    Preparation and Storage

    • Reconstitution

      Reconstitute at 0.2 mg/mL in sterile PBS.

    • Shipping

      The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

    • Stability & Storage

      Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

      • 12 months from date of receipt, -20 to -70 °C as supplied.

      • 1 month, 2 to 8 °C under sterile conditions after reconstitution.

      • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

    Background: ZNF281

    • Long Name:

      Zinc Finger Protein 281

    • Entrez Gene IDs:

      23528 (Human); 226442 (Mouse); 305083 (Rat)

    • Alternate Names:

      FLJ20557; GZP1; ZBP-99; zinc finger protein 416; ZNF281; ZNP-99


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