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Human/Mouse IkB-alpha Affinity Purified Polyclonal Ab 25 UG

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產(chǎn)品介紹

    基本參數(shù)

    詳細(xì)說明

    • Species Reactivity

      Human, Mouse

    • Specificity

      Detects human and mouse IkB-alpha in Western blots.

    • Source

      Polyclonal Sheep IgG

    • Purification

      Antigen Affinity-purified

    • Immunogen

      E. coli-derived recombinant mouse IkB-alpha    
      Met1-Leu314    
      Accession # Q9Z1E3

    • Formulation

      Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

    • Label

      Unconjugated

    Applications

    • Recommended    
      Concentration

      Sample

    • Western Blot

      0.1 μg/mL

      See below


    • Immunocytochemistry

      5-15 μg/mL

      See below


    Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

    Data Examples

    Western Blot      
         

    Detection of Human/Mouse IkB-alpha by Western Blot. Western blot shows lysates of NIH?3T3 mouse embryonic fibroblast cell line, DA3 mouse myeloma cell line, LNCaP human prostate cancer cell line, and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.1 μg/mL of Sheep Anti-Human/Mouse IkB-alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4299) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # ). A specific band was detected for IkB-alpha at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using .

    Immunocytochemistry      
         

    IkB? alpha in HeLa Human Cell Line. IkB? alpha was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human/Mouse IkB? alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4299) at 5 μg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights? 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # ) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for .

    Immunocytochemistry      
         

    IkB? alpha in HeLa Human Cell Line. IkB? alpha was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human/Mouse IkB? alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4299) at 5 μg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights? 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # ) and counterstained with DAPI (blue). View our protocol for .

    Preparation and Storage

    • Reconstitution

      Reconstitute at 0.2 mg/mL in sterile PBS.

    • Shipping

      The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

    • Stability & Storage

      Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

      • 12 months from date of receipt, -20 to -70 °C as supplied.

      • 1 month, 2 to 8 °C under sterile conditions after reconstitution.

      • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

    Background: IkB-alpha

    NF kappa B inhibitor alpha (IkB-alpha ) inhibits the NF kappa B complex by binding and sequestering it in the cytoplasm. Upon stimulation, IkB-alpha is phosphorylated on serine residues marking it for degradation by the ubiquitin pathway. Following IkB-alpha degradation, the NF kappa B complex is able to translocate to the nucleus and activate transcription.

    • Long Name:

      I-kappa-B-alpha

    • Entrez Gene IDs:

      4792 (Human); 18035 (Mouse); 25493 (Rat)

    • Alternate Names:

      alpha; I-kappa-B-alpha; IkappaBalphaNF-kappa-B inhibitor alpha; IKBAikB-alpha; IkBalpha; IkB-alpha; MAD3; MAD-3; Major histocompatibility complex enhancer-binding protein MAD3; NFKBI; NFKBIA; nuclear factor of kappa light chain gene enhancer in B-cells; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor















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