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Human ADAMTS4 Affinity Purified Polyclonal Ab 25 UG

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產(chǎn)品介紹

    基本參數(shù)

    詳細(xì)說明

    • Species Reactivity

      Human

    • Specificity

      Detects human ADAMTS4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human (rh) ADAMTS5 is observed and less than 1% cross-reactivity with rhADAMTS13, rhADAMTS-L1.2, and rhADAMTS1 is observed.

    • Source

      Polyclonal Goat IgG

    • Purification

      Antigen Affinity-purified

    • Immunogen

      Chinese hamster ovary cell line CHO-derived recombinant human ADAMTS4    
      Phe213-Cys685    
      Accession # O75173

    • Formulation

      Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

    • Label

      Unconjugated

    Applications

    • Recommended    
      Concentration

      Sample

    • Western Blot

      0.1 μg/mL

      Recombinant Human ADAMTS4 (Catalog # )


    • Immunoprecipitation

      25 μg/mL

      Conditioned cell culture medium spiked with Recombinant Human ADAMTS4 (Catalog # ),


    Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

    Preparation and Storage

    • Reconstitution

      Reconstitute at 0.2 mg/mL in sterile PBS.

    • Shipping

      The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

    • Stability & Storage

      Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

      • 12 months from date of receipt, -20 to -70 °C as supplied.

      • 1 month, 2 to 8 °C under sterile conditions after reconstitution.

      • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

    Background: ADAMTS4

    ADAMTS4 (a disintegrin and metalloproteinase with thrombospondin motifs 4), also known as aggrecanase-1, is a member of the family of secreted zinc proteases with a multi-domain structure (1?3). The protein precursors consist of a signal peptide and the following domains: pro, catalytic, disintegrin-like, TS type 1 motif, cysteine-rich, and spacer. It is the only ADAMTS identified that has one TS type I motif. It is an active protease effectively cleaving alpha -2-macroglobulin and aggrecan at multiple sites, and is inhibited by TIMP-3 with inhibition constants in subnanomolar range (4?6). It receives great attention due to the elevation in its mRNA level after treatment with Interleukin-1 (7). However, in a mouse model of osteoarthritis, ADAMTS4 knock-out mice did not exhibit any significant protective effect (8). The purified rhADAMTS4 starts at the catalytic domain and ends before the spacer domain. If desired, the aggrecanase activity can be inhibited by 5 mM 1,10?phenanthroline and recombinant human TIMP-3 (R&D Systems, Catalog # 973-TM).

    • References:

      1. Totorella, M. D. et al. (1999) Science 284:1664.

      2. Porter, S. et al. (2005) Biochem. J. 386:15.

      3. Nagase, H. and M. Kashiwagi (2003) Arthritis Res. Ther. 5:94.

      4. Tortorella, M. D. et al. (2004) J. Biol. Chem. 279:17554.

      5. Struglics, A. et al. (2006) Osteoarthritis Cartilage. 14:101.

      6. Kashiwagi, M. et al. (2001) J. Biol. Chem. 276:12501.

      7. Pratta, M. A. et al. (2003) Arthritis Reum. 48:119.

      8. Glasson, S. S. et al. (2004) Arthritis Reum. 50:2547.

    • Long Name:

      A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 4

    • Entrez Gene IDs:

      9507 (Human); 66015 (Rat)

    • Alternate Names:

      a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 4; ADAM metallopeptidase with thrombospondin type 1 motif, 4; ADAM-TS 4; ADAMTS2; ADAMTS-2; ADAMTS4; ADAM-TS4; ADAMTS-4; ADMP-1; ADMP-1EC 3.4.24.82; Aggrecanase 1; aggrecanase-1; EC 3.4.24; KIAA0688A disintegrin and metalloproteinase with thrombospondin motifs 4




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