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Human Crk Affinity Purified Polyclonal Ab 25 UG

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Human Crk Affinity Purified Polyclonal Ab 25 UG信息二維碼

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Anti-PIM2 antibody [EPR6988]  10μl Anti-PIM2 antibody [EPR6988]  100μl Anti-PIM2 antibody [EPR6987]  10μl Anti-PIM2 antibody [EPR6987]  40μl Anti-PIM2 antibody [EPR6987]  100μl Anti-Pigu antibody [EPR16424] - C-terminal  10μl

產(chǎn)品介紹

    基本參數(shù)

    詳細說明

    • Species Reactivity

      Human

    • Specificity

      Detects human Crk in Western blots.

    • Source

      Polyclonal Sheep IgG

    • Purification

      Antigen Affinity-purified

    • Immunogen

      E. coli-derived recombinant human Crk    
      Gln205-Ser304    
      Accession # P46108

    • Formulation

      Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

    • Label

      Unconjugated

    Applications

    • Recommended    
      Concentration

      Sample

    • Western Blot

      1 μg/mL

      See below


    Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

    Data Examples

    Western Blot      
         

    Detection of Human Crk by Western Blot. Western blot shows lysates of BaF3 mouse pro?B cell line, NS0 mouse myeloma cell line, K562 human chronic myelogenous leukemia cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 μg/mL of Human Crk Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4316) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # ). A specific band was detected for Crk at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using .

    Preparation and Storage

    • Reconstitution

      Reconstitute at 0.2 mg/mL in sterile PBS.

    • Shipping

      The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

    • Stability & Storage

      Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

      • 12 months from date of receipt, -20 to -70 °C as supplied.

      • 1 month, 2 to 8 °C under sterile conditions after reconstitution.

      • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

    Background: Crk

    Crk belongs to a family of adaptor molecules that are widely expressed in numerous tissues. It was originally isolated from a chicken tumor sample, and despite lacking enzymatic activity, this protein is involved in many signal transduction pathways leading to phosphorylation of multiple proteins. Crk forms multi protein complexes via it’s SH2 and SH3 domains leading to activation of downstream molecules involved in growth, differentiation, cell adhesion and migration.

    • Long Name:

      V-Crk Sarcoma Virus CT10 Oncogene Homolog

    • Entrez Gene IDs:

      1398 (Human); 12928 (Mouse); 54245 (Rat)

    • Alternate Names:

      adapter molecule crk; avian sarcoma virus CT10 (v-crk) oncogene homolog; Crk; CRKII; p38; Proto-oncogene c-Crk; v-crk avian sarcoma virus CT10 oncogene homolog; v-crk sarcoma virus CT10 oncogene homolog (avian)










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