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詳細(xì)說明
概述
產(chǎn)品名稱Anti-NPHS2抗體[EPR13820]
描述
兔單克隆抗體[EPR13820] to NPHS2
經(jīng)測試應(yīng)用IHC-P,WB
種屬反應(yīng)性
與反應(yīng): Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NPHS2 aa 350 to the C-terminus. The exact sequence is proprietary.
Database link: Q9NP85陽性對照
Human fetal kidney lysate; Human kidney tissue, human skeletal muscle tissue. Rat and mouse kidney.
常規(guī)說明
This product is a recombinant rabbit monoclonal antibody.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Produced using Abcam's RabMAb? technology. RabMAb? technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.
性能
形式Liquid
存放說明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存儲溶液Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA純度Protein A purified
克隆單克隆
克隆編號EPR13820
同種型IgG
研究領(lǐng)域
Cell Biology
Other Antibodies
Other Antibodies
Tags & Cell Markers
Cell Type Markers
Other Cell Types
Signal Transduction
Adapters
Transmembrane
Anti-NPHS2 antibody [EPR13820] 圖像
![Western blot - Anti-NPHS2 antibody [EPR13820] (ab181143)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181143WB2.jpg)
Western blot - Anti-NPHS2 antibody [EPR13820] (ab181143)
Anti-NPHS2 antibody [EPR13820] (ab181143) at 1/10000 dilution + Human fetal kidney at 20 μg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 42 kDa
![Western blot - Anti-NPHS2 antibody [EPR13820] (ab181143)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181143143.jpg)
Western blot - Anti-NPHS2 antibody [EPR13820] (ab181143)
Anti-NPHS2 antibody [EPR13820] (ab181143) at 1/50000 dilution (unpurified) + Human fetal kidney lysate at 20 μg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution
Predicted band size : 42 kDa
Observed band size : 42 kDa
![Western blot - Anti-NPHS2 antibody [EPR13820] (ab181143)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181143WB1.jpg)
Western blot - Anti-NPHS2 antibody [EPR13820] (ab181143)
All lanes : Anti-NPHS2 antibody [EPR13820] (ab181143) at 1/2000 dilution
Lane 1 : Mouse kidney
Lane 2 : Rat kidney
Lysates/proteins at 20 μg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 42 kDa
Additional bands at : 42 kDa. We are unsure as to the identity of these extra bands.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHS2 antibody [EPR13820] (ab181143)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181143HC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHS2 antibody [EPR13820] (ab181143)
ab181143 staining NPHS2 in human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.
Negative control 1: PBS in place of primary antibody.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHS2 antibody [EPR13820] (ab181143)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181143HC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHS2 antibody [EPR13820] (ab181143)
ab181143 staining NPHS2 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.
Negative control 1: PBS in place of primary antibody.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHS2 antibody [EPR13820] (ab181143)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab18114343b.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHS2 antibody [EPR13820] (ab181143)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NPHS2 with unpurified ab181143 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
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