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Human Proteinase 3/PRTN3 Affinity Purified Polyclonal Ab 25 UG

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Human Proteinase 3/PRTN3 Affinity Purified Polyclonal Ab 25 UG信息二維碼

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Anti-PRC1 (phospho T481) antibody [EP1514Y]  10μl Anti-PRC1 (phospho T481) antibody [EP1514Y]  40μl Anti-PRC1 (phospho T481) antibody [EP1514Y]  100μl Anti-PRAS40 antibody [EPR6402]  10μl Anti-PRAS40 antibody [EPR6402]  40μl Anti-PRAS40 antibody [EPR6402]  100μl

產(chǎn)品介紹

    基本參數(shù)

    詳細(xì)說明

    • Species Reactivity

      Human

    • Specificity

      Detects human Proteinase 3/Myeloblastin/PRTN3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Cathepsin B and rhGranzyme B is observed.

    • Source

      Polyclonal Sheep IgG

    • Purification

      Antigen Affinity-purified

    • Immunogen

      S. frugiperda insect ovarian cell line     Sf 21-derived recombinant human Proteinase 3/Myeloblastin/PRTN3    
      Ala26-Arg249    
      Accession # P24158

    • Formulation

      Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

    • Label

      Unconjugated

    Applications

    • Recommended    
      Concentration

      Sample

    • Western Blot

      1 μg/mL

      See below


    • Simple Western

      10 μg/mL

      See below


    Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

    Data Examples

    Western Blot      
         

    Detection of Human Proteinase 3/Myeloblastin/
    PRTN3 by Western Blot.
    Western blot shows lysates of HL?60 human acute promyelocytic leukemia cell line, human peripheral blood lymphocytes (PBL), and human peripheral blood mononuclear cells (PBMC). PVDF Membrane was probed with 1 μg/mL of Sheep Anti-Human Proteinase 3/Myeloblastin/PRTN3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6134) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # ). A specific band was detected for Proteinase 3/Myeloblastin/
    PRTN3 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using .

    Simple Western      
         

    Detection of Human Proteinase 3/Myeloblastin/PRTN3 by Simple WesternTM. Simple Western lane view shows lysates of human peripheral blood mononuclear cell tissue, loaded at 0.2 mg/mL. A specific band was detected for Proteinase 3/Myeloblastin/PRTN3 at approximately 32 kDa (as indicated) using 10 μg/mL of Sheep Anti-Human Proteinase 3/Myeloblastin/PRTN3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6134) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # ). This experiment was conducted under reducing conditions and using the
    12-230 kDa separation system.

    Preparation and Storage

    • Reconstitution

      Reconstitute at 0.2 mg/mL in sterile PBS.

    • Shipping

      The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

    • Stability & Storage

      Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

      • 12 months from date of receipt, -20 to -70 °C as supplied.

      • 1 month, 2 to 8 °C under sterile conditions after reconstitution.

      • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

    Background: Proteinase 3/Myeloblastin/PRTN3

    Proteinase 3 (PRTN3), also known as Myeloblastin (MBN), is a 32-33 kDa member of the peptidase S1 family of enzymes. It is expressed by monocytes and neutrophils, the latter of which either secretes it, sequesters it in azurophilic granules, or expresses it on the cell surface. When secreted, it acts on HK and activates the kinin pathway. In azurophilic granules, it aids in the digestion of phagocytosed material. On the cell surface, it likely acts on ECM. Human PRTN3 proprecursor is 231 amino acids (aa) in length. It contains an Ala26Glu27 propeptide that is removed during maturation, a 221 aa mature enzyme (aa 28-248), and an eight aa C?terminal propeptide (aa 249-256). Within the cell, a 35 kDa immature form exists; on the cell surface, both constitutively inactive, and induced active forms may be found, often in a noncovalent association with CD177/NB1. Over aa 26-249, human PRTN3 shares 68% aa identity with mouse PRTN3.

    • Entrez Gene IDs:

      5657 (Human); 19152 (Mouse); 314615 (Rat)

    • Alternate Names:

      ACPA; AGP7; AGP7PR3; azurophil granule protein 7; C-ANCA; EC 3.4.21; EC 3.4.21.76; Leukocyte proteinase 3; MBN; MBT; Myeloblastin; Neutrophil proteinase 4; NP4; NP-4; P29C-ANCA antigen; PR-3CANCA; Proteinase 3; PRTN3; serine proteinase, neutrophil; Wegener autoantigen; Wegener granulomatosis autoantigen





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