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Human RARRES3 Affinity Purified Polyclonal Ab 25 UG

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Human RARRES3 Affinity Purified Polyclonal Ab 25 UG信息二維碼

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Anti-PRC1 (phospho T481) antibody [EP1514Y]  10μl Anti-PRC1 (phospho T481) antibody [EP1514Y]  40μl Anti-PRC1 (phospho T481) antibody [EP1514Y]  100μl Anti-PRAS40 antibody [EPR6402]  10μl Anti-PRAS40 antibody [EPR6402]  40μl Anti-PRAS40 antibody [EPR6402]  100μl

產(chǎn)品介紹

    基本參數(shù)

    詳細說明

    • Species Reactivity

      Human

    • Specificity

      Detects human RARRES3 in direct ELISAs.  In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) RARRES1 and rhLatexin is observed.

    • Source

      Polyclonal Sheep IgG

    • Purification

      Antigen Affinity-purified

    • Immunogen

      E. coli-derived recombinant human RARRES3    
      Asp12-Lys134    
      Accession # Q9UL19

    • Formulation

      Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

    • Label

      Unconjugated

    Applications

    • Recommended    
      Concentration

      Sample

    • Immunocytochemistry

      5-15 μg/mL

      See below


    Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

    Data Examples

    Immunocytochemistry      
         

    RARRES3 in Raji Human Cell Line. RARRES3 was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Human RARRES3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6139) at 10 μg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights? 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # ) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for .

    Preparation and Storage

    • Reconstitution

      Reconstitute at 0.2 mg/mL in sterile PBS.

    • Shipping

      The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

    • Stability & Storage

      Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

      • 12 months from date of receipt, -20 to -70 °C as supplied.

      • 1 month, 2 to 8 °C under sterile conditions after reconstitution.

      • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

    Background: RARRES3

    RARRES3 (Retinoic acid receptor responder 3; also Tig3) is an 18-20 kDa member of the lecithin retinol acyltransferase family of molecules. It is widely expressed, particularly in keratinocytes, and is considered to have multiple functions. These include phospholipase A activity and an ability to both activate type I transglutaminase, and inhibit Ras activation Human RARRES3 is 164 amino acids (aa) in length. It contains a catalytic residue at Cys113, and a hydrophobic membrane-anchoring domain between aa 134-164. Within the cell, RARRES3 appears to be equally distributed between membrane and soluble fractions. RARRES3 is also reported to form higher order multimers. One potential isoform exists that shows a 26 aa substitution for aa 131-164. No meaningful structural ortholog of RARRES3 exists in rodent.

    • Long Name:

      Retinoic Acid Receptor Responder [Tazarotene-induced] 3

    • Entrez Gene IDs:

      5920 (Human)

    • Alternate Names:

      HRASLS4; PLA1/2-3; RARRES3; RAR-responsive protein TIG3; retinoic acid receptor responder (tazarotene induced) 3; retinoic acid receptor responder protein 3; retinoic acid-inducible gene 1; Retinoid-inducible gene 1 protein; RIG1; Tazarotene-induced gene 3 protein; TIG3; TIG3MGC8906



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