• Species Reactivity

  Human

• Specificity

  Detects human GFR alpha ?2/GDNF R alpha ?2 in direct ELISAs and Western blots. In direct ELISAs, approximately 75% cross?reactivity with recombinant mouse GFR alpha -2 is observed and less than 5% cross-reactivity with recombinant human GFR alpha -1 is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human GFR alpha ?2/GDNF R alpha ?2    
  Ser22-Ser441    
  Accession # O00451

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.1 μg/mL

  Recombinant Human GFR alpha ?2/GDNF R alpha ?2 Fc Chimera (Catalog # )

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• Immunohistochemistry

  5-15 μg/mL

  Immersion fixed paraffin-embedded sections of human dorsal root ganglia

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• Blockade of Receptor-ligand Interaction

  In a functional ELISA, 2-6 μg/mL of this antibody will block 50% of the binding of 2 ng/mL of Recombinant Human GDNF (Catalog # ) to immobilized Recombinant Human GFR alpha -2 Fc Chimera (Catalog # ) coated at 1 μg/mL (100 μL/well). At 20 μg/mL, this antibody will block >90% of the binding.

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Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GFR alpha-2/GDNF R alpha-2

Glial cell line-derived growth factor (GDNF), neurturin (NTN), persephin (PSP) and artemin, distant members of theTGF-beta  superfamily, are neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits (GDNF receptor alpha -1 [GFR alpha -1], also known as Trn R1 or GFR alpha -2, also known as Trn R2). GFR alpha -1 and -2 are members of a family of at least four cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF or NTN to membrane-associated GFR alpha -1 or GFR alpha -2 initiates the association with and activation of the Ret tyrosine kinase.

Human GFR alpha -2 cDNA encodes a 464 amino acid (aa) residue protein with a putative N-terminal 21 aa residue hydrophobic signal peptide. Like other GPI-linked proteins, human GFR alpha -2 has a C-terminal hydrophobic region which is preceded by a three aa residue (GPS) GPI-binding site. Human GFR alpha -2 shares 96.5% amino acid identity with mouse GFR alpha -2. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors.

• References:

1. Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117.

2. Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.

3. Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.

4. Baloh, R.H. et al. (1998) Neuron 21:1291.

• Long Name:

  Glial Cell line-derived Neurotrophic Factor Receptor alpha 2

• Entrez Gene IDs:

  2675 (Human); 14586 (Mouse)

• Alternate Names:

  GDNF family receptor alpha 2; GDNF family receptor alpha-2; GDNF R alpha-2; GDNF receptor alpha-2; GDNF receptor beta; GDNFR-alpha-2; GDNFR-beta; GDNFRBRET ligand 2; GFR alpha2; GFR alpha-2; GFRA2; GFRa-2; GFR-alpha 2; GFR-alpha-2; glial cell line derived neurotrophic factor receptor, beta; Neurturin receptor alpha; NRTNR-alpha; NTNRA; NTNR-alpha; PI-linked cell-surface accessory protein; RETL2TGF-beta-related neurotrophic factor receptor 2; TGF-beta related neurotrophic factor receptor 2; TRN receptor, GPI-anchored; TRNR2NRTNR-ALPHA