• Species Reactivity

  Mouse

• Specificity

  Detects mouse beta IG-H3 in ELISAs and Western blots. In sandwich immunoassays, less than 6% cross-reactivity with recombinant human (rh)  beta IG-H3 is observed.

• Source

  Polyclonal Sheep IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse beta IG-H3    
  Gly24-His683    
  Accession # P82198

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.1 μg/mL

  Recombinant Mouse beta IG-H3 (Catalog # )

• 
  

• Immunohistochemistry

  5-15 μg/mL

  See below

• 
  

• Mouse beta IG-H3 Sandwich Immunoassay

  Reagent    

• ELISA Capture (Matched Antibody Pair)

  0.2-0.8 μg/mL 

  Mouse beta IG?H3 Antibody (Catalog # )

• ELISA Detection (Matched Antibody Pair)

  0.1-0.4 μg/mL 

  Mouse beta IG?H3 Biotinylated Antibody (Catalog # )

• ELISA Standard

   

  Recombinant Mouse beta IG-H3 Protein, CF (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta IG-H3

Beta IG-H3, also known as TGFBI and RGD-CAP, is a matricellular adaptor protein that is induced in most cell types in response to TGF-beta stimulation (1?4). The mouse beta IG-H3 cDNA encodes a 683 amino acid (aa) precursor that includes a 23 aa signal sequence, one EMI domain, four FAS1 domains, and one RGD motif (2). Mouse beta IG-H3 shares 91% aa sequence identity with human and porcine beta IG-H3. beta IG-H3 is expressed as a 75 kDa protein with no post-translational additions (5). Following secretion, cleavages at multiple positions near the C-terminal end liberate peptides with pro-apoptotic activity (5, 6). Peptides that encompass the RGD motif contribute to the pro-apoptotic effects of TGF-beta (6). FAS1 domains contain YH motifs that are characterized by conserved Tyr and His residues (7). The YH motifs in each of the FAS1 domains enable beta IG-H3 to bind to matrix fibronectin, collagen I, collagen VI, biglycan, and decorin (3, 8?11), in addition to cell expressed integrins alpha V/ beta 3, alpha V beta 5, and alpha 3 beta 1 (7, 8, 12, 13). The expression of beta IG-H3 is modulated at particular developmental stages in some cell types. It is upregulated in keratinocytes and immature dendritic cells but downregulated in osteoblasts (8, 12, 14). It promotes keratinocyte differentiation but blocks osteoblast differentiation (8, 12). beta IG-H3 stimulates macrophage endocytosis and vascular endothelial cell proliferation and migration (13, 14). High glucose levels induce beta IG-H3 in renal proximal tubule cells which is predictive of diabetic nephropathy (3). Several point mutations (clustered in the fourth FAS1 domain) of beta IG-H3 are linked to different corneal dystrophies (15). beta IG-H3 is downregulated in many cancers (4, 16) and functions as a suppressor of tumorigenicity when overexpressed (2, 4, 16).

• References:

1. Skonier, J. et al. (1992) DNA Cell Biol. 11:511.

2. Skonier, J. et al. (1994) DNA Cell Biol. 13:571.

3. Lee, S-H. et al. (2003) Kidney Int. 64:1012.

4. Zhao, Y.L. et al. (2002) Oncogene 21:7471.

5. Andersen, R.B. et al. (2004) Biochemistry 43:16374.

6. Kim, J-E. et al. (2003) Oncogene 22:2045.

7. Kim, J-E. et al. (2002) J. Biol. Chem. 277:46159.

8. Thapa, N. et al. (2005) Bone 36:232.

9. Hanssen, E. et al. (2003) J. Biol. Chem. 278:24334.

10. Billings, P.C. et al. (2002) J. Biol. Chem. 277:28003.

11. Reinboth, B. et al. (2006) J. Biol. Chem. 281:7816.

12. Oh, J-E. et al. (2005) J. Biol. Chem. 280:21629.

13. Nam, J-O. et al. (2003) J. Biol. Chem. 278:25902.

14. Cao, W. et al. (2006) Blood 107:2777.

15. Stewart, H.S. et al. (1999) Hum. Mutat. 14:126.

16. Zhao, Y. et al. (2006) Mol. Carcinog. 45:84.

• Long Name:

  TGF-beta Induced Gene H3

• Entrez Gene IDs:

  7045 (Human); 21810 (Mouse)

• Alternate Names:

  beta IGH3; beta IG-H3; BIGH3EBMD; CDB1; CDG2; CDGG1transforming growth factor, beta-induced, 68kD; CSD; CSD1; CSD2; CSD3; Kerato-epithelin; LCD1; RGD-CAP; RGD-containing collagen-associated protein; TGFBI; TGFBIP; transforming growth factor, beta-induced, 68kDa; transforming growth factor-beta-induced protein ig-h3