• Species Reactivity

  Mouse

• Specificity

  Detects mouse ASCL1/Mash1 in direct ELISAs and Western blots.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant mouse ASCL1/Mash1    
  His74-Asp173 (Gln160Glu)    
  Accession # Q53WV9

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.1 μg/mL

  Recombinant Mouse ASCL1/Mash1

• 
  

• Immunohistochemistry

  5-15 μg/mL

  Immersion fixed frozen sections of mouse embryo (E13.5-15.5) and thymus

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ASCL1/Mash1

Mash1, also known as achaete-scute complex-like 1 (ASCL1), is a member of the basic helix-loop-helix family of transcription factors. It is required for neuronal commitment and differentiation. The mouse immunogen used to generate this antibody shares 97% amino acid sequence identity with the equivalent region of human Mash1.

• Long Name:

  Achaete Scute Complex-like 1

• Entrez Gene IDs:

  429 (Human); 17172 (Mouse)

• Alternate Names:

  achaete-scute complex (Drosophila) homolog-like 1; achaete-scute complex homolog 1 (Drosophila); achaete-scute complex-like 1 (Drosophila); achaete-scute homolog 1; ASCL1; ASH1; ASH-1; ASH1MASH1; BHLHA46; bHLHa46achaete-scute complex-like 1; Class A basic helix-loop-helix protein 46; HASH1; HASH1achaete scute protein; Mash1