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Human MAO-A Affinity Purified Polyclonal Ab 25 UG

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Human MAO-A Affinity Purified Polyclonal Ab 25 UG信息二維碼

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產(chǎn)品介紹

    基本參數(shù)

    詳細(xì)說(shuō)明

    • Species Reactivity

      Human

    • Specificity

      Detects human MAO-A in direct ELISAs and Western blots. 

    • Source

      Polyclonal Sheep IgG

    • Purification

      Antigen Affinity-purified

    • Immunogen

      S. frugiperda insect ovarian cell line     Sf21-derived recombinant human MAO-A    
      Met1-Ser497 (predicted)    
      Accession # P21397

    • Formulation

      Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

    • Label

      Unconjugated

    Applications

    • Recommended    
      Concentration

      Sample

    • Western Blot

      0.25 μg/mL

      See below


    Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

    Data Examples

    Western Blot      
         

    Detection of Human MAO-A by Western Blot. Western blot shows lysates of human placenta tissue, human liver tissue, and human kidney tissue. PVDF membrane was probed with 0.25 μg/mL of Sheep Anti-Human MAO-A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8247) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # ). A specific band was detected for MAO-A at approximately 60-65 kDa (as indicated). This experiment was conducted under reducing conditions and using .

    Preparation and Storage

    • Reconstitution

      Reconstitute at 0.2 mg/mL in sterile PBS.

    • Shipping

      The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

    • Stability & Storage

      Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

      • 12 months from date of receipt, -20 to -70 °C as supplied.

      • 1 month, 2 to 8 °C under sterile conditions after reconstitution.

      • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

    Background: MAO-A

    MAO-A (Monoamine oxidase type A and Amine oxidase [flavin-containing] A)  is a 60 kDa (predicted) member of the flavin monoamine oxidase family of proteins.  MAO- A is an enzyme that degrades amine neurotransmitters, such as dopamine, norepinephrine, and serotonin. It localizes to the outer mitochondrial membrane. Mutation in this gene results in monoamine oxidase deficiency, or Brunner syndrome, a form of X-linked non-dysmorphic mild mental retardation. Male patients are affected by borderline mental retardation and exhibit abnormal behavior, including disturbed regulation of impulsive aggression. Obligate female carriers have normal intelligence and behavior. Over aa 1-497, human MOA-A shares 88% aa sequence identity with mouse MOA-A.

    • Long Name:

      Monoamine Oxidase A

    • Entrez Gene IDs:

      4128 (Human); 17161 (Mouse); 29253 (Rat)

    • Alternate Names:

      amine oxidase [flavin-containing] A; EC 1.4.3; EC 1.4.3.4; MAOA; MAO-A; monoamine oxidase A; Monoamine oxidase type A






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