• Species Reactivity

  Human

• Specificity

  Detects human Integrin beta 1/CD29.

• Source

  Monoclonal Mouse IgG    ₁ Clone # P5D2

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Human skin keratinocytes

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25 μg/10    ⁶ cells

  See below

• 
  

• Immunoprecipitation

  Lin, Q. et al. (2004) Biochim. Biophys. Acta 1689:175.
  Joneckis, C.C. et al. (1993) Blood 82:3548.
  

• 
  

• Blockade of Receptor-ligand Interaction

  Mukhopadhyay, N.K.     et al. (2004) Ann. Thorac. Surg.     78:450.    
  Blaschke, F.     et al. (2002) Biochem. Biophys. Res. Commun.     296:890.

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

• Immunocytochemistry

  8-25 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 1/CD29

The Integrin beta 1 subunit, also known as CD29, associates with at least ten different Integrin alpha subunits. It regulates not only its multiple ligands, but activates a signaling cascade in its expressing cells. CD29 is proposed to play a role in cell adhesion, apoptosis, and differentiation (1,2). Over amino acids (aa) 21-728, human and mouse share 92% aa sequence identity.

• References:

1. Barkan, D. and A.F. Chambers (2011) Clin. Cancer Res. 17:7219.

2. Humphries, M.J. (2000) Biochem. Soc. Trans. 28:311.

• Entrez Gene IDs:

  3688 (Human); 16412 (Mouse)

• Alternate Names:

  CD29 antigen; CD29; Fibronectin receptor subunit beta; FNRBVLAB; GPIIA; Integrin beta 1; integrin beta-1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12); ITGB1; MDF2; MSK12; very late activation protein, beta polypeptide; VLA-4 subunit beta; VLA-BETA