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Human CX3CL1/Fractalkine MAb (Clone 81506) 25 UG

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Human CX3CL1/Fractalkine MAb (Clone 81506) 25 UG信息二維碼

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產(chǎn)品介紹

    基本參數(shù)

    詳細(xì)說(shuō)明

    • Species Reactivity

      Human

    • Specificity

      Detects human CX3CL1/Fractalkine in ELISAs. In sandwich immunoassays, less than 10% cross-reactivity with recombinant mouse CXCL6 and less than 0.2% cross-reactivity with recombinant human (rh) CXCL6, rhCXCL9, and recombinant rat CX3CL1 is observed.    
       

    • Source

      Monoclonal Mouse IgG    1 Clone # 81506

    • Purification

      Protein A or G purified from hybridoma culture supernatant

    • Immunogen

      NS0-derived and     E. coli-derived Recombinant Human CX3CL1/Fracktalkine    
      Gln25-Arg339    
      Accession # P78423

    • Formulation

      Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

    • Endotoxin Level

      <0.10 EU per 1 μg of the antibody by the LAL method.  

    • Label

      Unconjugated

    Applications

    • Recommended    
      Concentration

      Sample

    • CyTOF-ready

      Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.


    • Intracellular Staining by Flow Cytometry

      2.5 μg/10    6 cells

      Human CX3CL1/Fractalkine transfected NS0 cells fixed with paraformaldehyde and permeabilized with saponin


      • Human CX3CL1/Fractalkine Sandwich Immunoassay

        Reagent    

    • ELISA Capture (Matched Antibody Pair)

      2-8 μg/mL 

      Human CX3CL1/Fractalkine Chemokine Domain Antibody (Catalog # )

    • ELISA Detection (Matched Antibody Pair)

      0.5-2.0 μg/mL 

      Human CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody (Catalog # )

    • ELISA Standard

       

      Recombinant Human CX3CL1/Fractalkine (Full Length) Protein (Catalog # )


    • Neutralization

      Measured by its ability to neutralize CX3CL1/Fractalkine-induced chemotaxis in the BaF3 mouse pro?B cell line transfected with mouse CX3CR1. The Neutralization Dose (ND     50) is typically 1-5 μg/mL in the presence of 100 ng/mL Recombinant Human CX3CL1/Fractalkine.

    Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

    Data Examples

    Neutralization      
         

    Chemotaxis Induced by CX3CL1/Fractalkine and Neutralization by Human CX3CL1/Fractalkine Antibody. Recombinant Human CX3CL1/Fractalkine (Catalog # ) chemoattracts the BaF3 mouse pro?B cell line transfected with mouse CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # ). Chemotaxis elicited by Recombinant Human CX3CL1/Fractalkine (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CX3CL1/Fractalkine Chemokine Domain Monoclonal Antibody (Catalog # MAB3652). The ND50 is typically 1-5 μg/mL.

    Preparation and Storage

    • Reconstitution

      Reconstitute at 0.5 mg/mL in sterile PBS.

    • Shipping

      The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

    • Stability & Storage

      Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

      • 12 months from date of receipt, -20 to -70 °C as supplied.

      • 1 month, 2 to 8 °C under sterile conditions after reconstitution.

      • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

    Background: CX3CL1/Fractalkine

    CX3CL1, also known as Fractalkine, is a type I membrane protein in which a chemokine domain possessing a unique C-X3-C cysteine motif is tethered on a long mucin-like stalk. It can also be released as a soluble molecule upon proteolysis at a membrane proximal site.

    • Entrez Gene IDs:

      6376 (Human); 20312 (Mouse); 89808 (Rat)

    • Alternate Names:

      ABCD-3; C3Xkine; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; CX3CL1; CXC3; CXC3C; FKN; Fractalkine; Neurotactin; neurotactin); NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine; NTTSmall-inducible cytokine D1; SCYD1C-X3-C motif chemokine 1; small inducible cytokine subfamily D (Cys-X3-Cys), member-1











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