• Species Reactivity

  Human

• Specificity

  Detects human CX3CL1/Fractalkine in ELISAs. In sandwich immunoassays, less than 10% cross-reactivity with recombinant mouse CXCL6 and less than 0.2% cross-reactivity with recombinant human (rh) CXCL6, rhCXCL9, and recombinant rat CX3CL1 is observed.    
   

• Source

  Monoclonal Mouse IgG    ₁ Clone # 81506

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  NS0-derived and     E. coli-derived Recombinant Human CX3CL1/Fracktalkine    
  Gln25-Arg339    
  Accession # P78423

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

• Intracellular Staining by Flow Cytometry

  2.5 μg/10    ⁶ cells

  Human CX3CL1/Fractalkine transfected NS0 cells fixed with paraformaldehyde and permeabilized with saponin

• 
  

• Human CX3CL1/Fractalkine Sandwich Immunoassay

  Reagent    

• ELISA Capture (Matched Antibody Pair)

  2-8 μg/mL 

  Human CX3CL1/Fractalkine Chemokine Domain Antibody (Catalog # )

• ELISA Detection (Matched Antibody Pair)

  0.5-2.0 μg/mL 

  Human CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody (Catalog # )

• ELISA Standard

   

  Recombinant Human CX3CL1/Fractalkine (Full Length) Protein (Catalog # )

• 
  

• Neutralization

  Measured by its ability to neutralize CX3CL1/Fractalkine-induced chemotaxis in the BaF3 mouse pro?B cell line transfected with mouse CX3CR1. The Neutralization Dose (ND     ₅₀) is typically 1-5 μg/mL in the presence of 100 ng/mL Recombinant Human CX3CL1/Fractalkine.

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CX3CL1/Fractalkine

CX3CL1, also known as Fractalkine, is a type I membrane protein in which a chemokine domain possessing a unique C-X3-C cysteine motif is tethered on a long mucin-like stalk. It can also be released as a soluble molecule upon proteolysis at a membrane proximal site.

• Entrez Gene IDs:

  6376 (Human); 20312 (Mouse); 89808 (Rat)

• Alternate Names:

  ABCD-3; C3Xkine; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; CX3CL1; CXC3; CXC3C; FKN; Fractalkine; Neurotactin; neurotactin); NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine; NTTSmall-inducible cytokine D1; SCYD1C-X3-C motif chemokine 1; small inducible cytokine subfamily D (Cys-X3-Cys), member-1