• Species Reactivity

  Human

• Specificity

  Detects human ErbB3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows no cross-reactivity with recombinant human (rh) EGF R, rhErbB4, or rhErbB2.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 66219

• Purification

  Protein A or G purified from ascites

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human ErbB3    
  Ser20-Thr643    
  Accession # P21860

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 μg/mL

  Recombinant Human ErbB3/Her3 Fc Chimera (Catalog # )

• 
  

• Immunohistochemistry

  8-25 μg/mL

  Immersion fixed paraffin-embedded sections of human lung, human lung cancer tissue, and human breast

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ErbB3/Her3

ErbB3, also called Her3 (human epidermal growth factor receptor 3), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. Among ErbB family members, ErbB3 is unique in that it contains a defective kinase domain. ErbB3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. Monomeric ErbB3 serves as a low affinity receptor for the heregulins (HRG). ErbB3 heterodimerizes with ErbB2 to form a high affinity receptor complex. In contrast, ErbB3 homodimerization or heterodimerization with ErbB4 forms a low affinity heregulin-binding complex. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. The cytoplasmic domain of ErbB3 also contains six consensus binding motifs for the SH2 domain of the regulatory p85 subunit of phosphoinositide 3-kinase (PI 3-kinase, PI3K) as well as one proline-rich consensus binding motif for the SH3 domain of p85. Human ErbB3 consists of 1342 amino acids (aa) with a 19 aa signal sequence, a 624 aa extracellular domain, a 21 aa transmembrane region, and a 678 aa cytoplasmic domain. ErbB3 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation

• References:

1. Kraus, M.H. et. al. (1989) Proc. Natl. Acad. Sci. 86:9193.

2. Plowman, G.D. et. al. (1990) Proc. Natl. Acad. Sci. USA 87:4905.

3. Carraway, K.L. 3rd et. al. (1994) J. Biol. Chem. 269:14303.

4. Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.

5. Sundaresan, S. et. al. (1998) Endocrinology 139:4756.

6. Hellyer, N.J. et. al. (1998) Biochem. J. 333:757.

7. Schaefer, G. et. al. (1999) J. Biol. Chem. 274:859.

8. Hellyer, N.J. et. al. (2001) J. Biol. Chem. 276:42153.

9. Schlessinger, J. (2000) Cell 103:211.

10. Daly, R.J. (1999) Growth Factors 16:255.

• Long Name:

  Receptor Tyrosine Protein Kinase ErbB3

• Entrez Gene IDs:

  2065 (Human); 13867 (Mouse)

• Alternate Names:

  c-erbB3; EC 2.7.10; EC 2.7.10.1; ErbB3; ErbB-3; erbB3-S; HER3; HER3c-erbB-3; LCCS2; lethal congenital contracture syndrome 2; MDA-BF-1; MGC88033; p180-ErbB3; p45-sErbB3; p85-sErbB3; Proto-oncogene-like protein c-ErbB-3; receptor tyrosine-protein kinase erbB-3; Tyrosine kinase-type cell surface receptor HER3; v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)