• Species Reactivity

  Mouse

• Specificity

  Detects mouse Desert Hedgehog/Dhh N-Terminus in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant mouse (rm) Shh N-terminal peptide (aa 25?198) is observed. Additionally, in direct ELISAs, no cross?reactivity with rmDhh C-terminal peptide (aa 199?396) and rmIhh C-terminal peptide (aa 241?449) is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant mouse Desert Hedgehog/Dhh    
  aa 23-198

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.1 μg/mL

  Recombinant Mouse Desert Hedgehog (C23II), N-Terminus (Catalog # )

• 
  

• Immunohistochemistry

  5-15 μg/mL

  Perfusion fixed frozen sections of mouse spinal cord (dorsal roots)

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Desert Hedgehog/Dhh

Dhh regulates testis development and spermatogenesis via interaction of its N-terminal domain with the receptors Patched and Patched-2. Hedgehog proteins are secreted as precursor molecules that undergo autocatalytic internal cleavage to yield an N-terminal domain that has signaling activity and a C-terminal domain that is active in precursor processing.

• Entrez Gene IDs:

  50846 (Human); 13363 (Mouse)

• Alternate Names:

  desert hedgehog (Drosophila) homolog; desert hedgehog homolog; desert hedgehog protein; Desert Hedgehog; DHH; HHG-3MGC35145