• Species Reactivity

  Mouse

• Specificity

  Detects mouse sFRP-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) sFRP-1, recombinant mouse sFRP-3, or rhsFRP-4.

• Source

  Monoclonal Rat IgG    ₁ Clone # 331022

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse sFRP-2    
  Leu25-Cys295    
  Accession # P97299

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 μg/mL

  Recombinant Mouse sFRP?2 (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: sFRP-2

Secreted Frizzled Related Proteins (sFRPs) are a family of vertebrate proteins which contain homology to the ligand-binding domain of the Frizzled family of transmembrane receptors. The sFRPs are approximately 30-35 kDa in size and are comprised of 3 domains: a signal sequence; a cysteine-rich domain (CRD) of about 110 amino acids (aa) with a high degree of similarity to the Frizzled proteins, including 10 conserved cysteines, and a 175 aa conserved hydrophilic carboxy terminal region. Because sFRPs contain a CRD very similar to the region responsible for binding Wnt ligands in Frizzleds, sFRPS are thought to act as soluble antagonists of Wnt signals. sFRP-2, also known as SARP-1, SDF-5, and FRP-2, is expressed during mouse embryogenesis in the eye, brain, neural tube, craniofacial mesenchyme, joints, testis, pancreas, kidney, and regions of smooth muscle cell development. Expression in the adult animal has been detected in the eye, heart, lung, along with preadipose and adipose tissues in mice and humans. Mouse and human sFRP-2 proteins share 98% aa identity, and related proteins have also been identified in chick, rabbit, and frog. A variety of activities have been reported for sFRP-2 including an increased resistance to apoptosis, antagonism of Xwnt -8 signaling in Xenopus, regulation of Wnt-4 signaling (with sFRP-1) in renal organogenesis, and inhibition of the migration of glioma cells (1-7).

• References:

1. Rattner, A. et al. (1997) Proc. Natl. Acad. Sci. USA 94:2859.

2. Melkonyan, H. et al. (1997) Proc. Natl. Acad. Sci. USA 94:13636.

3. Hu, E. et al. (1998) BBRC 247:287.

4. Leimeister, C. et al. (1998) Mech. Dev. 75:29.

5. Roth, W. et al. (2000) Oncogene 19:4210.

6. Ladher, R. et al. (2000) Dev. Biol. 218:183.

7. Yoshino, K. et al. (2001) Mech. Dev. 102:45.

• Long Name:

  Secreted Frizzled Related Protein 2

• Entrez Gene IDs:

  6423 (Human); 20319 (Mouse)

• Alternate Names:

  FRP2; FRP-2secreted apoptosis related protein 1; SARP-1; SARP1sFRP-2; SDF-5; Secreted apoptosis-related protein 1; secreted frizzled-related protein 2; sFRP2; sFRP-2