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Human Lymphotoxin-alpha/TNF-beta Affinity Purified PAb 25 UG

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Human Lymphotoxin-alpha/TNF-beta Affinity Purified PAb 25 UG信息二維碼

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產(chǎn)品介紹

    基本參數(shù)

    詳細(xì)說明

    • Species Reactivity

      Human

    • Specificity

      Detects human Lymphotoxin? alpha /TNF? beta in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) TNF-alpha, rhsTNF RI and rhsTNF RII is observed.

    • Source

      Polyclonal Goat IgG

    • Purification

      Antigen Affinity-purified

    • Immunogen

      E. coli-derived recombinant human Lymphotoxin? alpha /TNF? beta    
      Leu35-Leu205    
      Accession # P01374

    • Formulation

      Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

    • Endotoxin Level

      <0.10 EU per 1 μg of the antibody by the LAL method.  

    • Label

      Unconjugated

    Applications

    • Recommended    
      Concentration

      Sample

    • Western Blot

      0.1 μg/mL

      Recombinant Human Lymphotoxin? alpha /TNF? beta (Catalog # )


    • Neutralization

      Measured by its ability to neutralize Lymphotoxin? alpha /TNF? beta -induced cytotoxicity in the L?929 mouse fibroblast cell line [Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J.     et al. (eds): IRL Press. 221]. The Neutralization Dose (ND    50) is typically 0.01-0.03 μg/mL in the presence of 0.25 ng/mL Recombinant Human Lymphotoxin? alpha /TNF? beta and 1 μg/mL actinomycin D.

    Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

    Data Examples

    Neutralization      
         

    Cytotoxicity Induced by Lymphotoxin? alpha /TNF? beta  and Neutralization by Human Lymphotoxin? alpha /TNF? beta Antibody. Recombinant Human Lymphotoxin? alpha /TNF? beta (Catalog # ) induces cytotoxicity in the the L?929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Human Lymphotoxin-alpha /TNF-beta (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Lymphotoxin? alpha /TNF? beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-211-NA). The ND50 is typically 0.01?0.03 μg/mL in the presence of the metabolic inhibitor actinomycin D (1 μg/mL).


    Preparation and Storage

    • Reconstitution

      Reconstitute at 0.2 mg/mL in sterile PBS.

    • Shipping

      The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

    • Stability & Storage

      Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

      • 12 months from date of receipt, -20 to -70 °C as supplied.

      • 1 month, 2 to 8 °C under sterile conditions after reconstitution.

      • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

    Background: Lymphotoxin-alpha/TNF-beta

    Tumor necrosis factor beta (TNF-beta ), also known as lymphotoxin-alpha (LT-alpha ), and TNF-alpha, are two structurally and functionally related proteins that bind to the same cell surface receptors (TNF RI and TNF RII) and produce a vast range of similar, but not identical, effects. Among these effects is the ability to kill certain tumor cells directly, from which the names tumor necrosis factor and lymphotoxin both derive. Mature TNF-beta /LT-alpha and TNF-alpha share approximately 35% protein sequence homology and the biologically active secreted forms of both proteins are homotrimers. Whereas TNF-alpha can exist as a type II membrane protein, TNF-beta /LT-alpha possesses a typical signal peptide sequence and is a secreted protein. It has been shown that TNF-beta /LT-alpha is also present on the cell surface of activated T, B, and LAK cells as a heteromeric complex with LT-beta, a type II membrane protein that is another member of the TNF ligand family. The genes for TNF-alpha, TNF-beta /LT-alpha, and LT-beta are closely linked within the major histocompatibility complex.

    TNF-beta /LT-alpha is expressed in activated T- and B-lymphocytes. In addition to its cytotoxic action on tumor cells, TNF-beta /LT-alpha has been shown to be a mediator of inflammation and immune function. Evidence is also accumulating that TNF-beta /LT-alpha and TNF-alpha are mediators in the pathogenesis of certain autoimmune diseases. TNF-beta /LT-alpha has also been shown to have a role in lymphoid organ development. Human and mouse TNF-beta /LT-alpha share approximately 74% homology in their amino acid sequence and exhibit cross-species activity.

    • Long Name:

      Tumor Necrosis Factor beta

    • Entrez Gene IDs:

      4049 (Human); 16992 (Mouse)

    • Alternate Names:

      LT; LTA; LT-alpha; lymphotoxin alpha (TNF superfamily, member 1); Lymphotoxin alpha; Lymphotoxinalpha; Lymphotoxin-alpha; tnfb; TNF-beta; TNFBlymphotoxin-alpha; TNFSF1; TNFSF1B; TNFSF1TNF-beta; tumor necrosis factor beta; Tumor necrosis factor ligand superfamily member 1












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