• Species Reactivity

  Human

• Specificity

  Detects human or mouse Pro Meprin beta Subunit/MEP1B in Western blots. It does not react with the mature form.

• Source

  Monoclonal Rat IgG    _2A Clone # 289703

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Human embryonic kidney cell line HEK293-derived recombinant human Meprin beta Subunit/MEP1B    
  Thr23-Ser593    
  Accession # Q16820

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 μg/mL

  Recombinant Human Meprin  beta  Subunit/MEP1B (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Meprin beta Subunit/MEP1B

Meprins are multimeric proteases composed of alpha and beta  subunits, which are members of the astacin family of zinc endopeptidases (1, 2). Both subunits form disulfide?linked homo- or heterooligomers, which are also referred to as Meprin A (composed of alpha  subunits with or without beta  subunits) and Meprin B (composed of beta  subunits only) (3). Although the two subunits share 42% identity in their amino acid sequence, they differ significantly in their oligomeric structure, post-translational processing and subsequently cellular location, and substrate and peptide bond specificity (4). The 701 aa sequence of the human Meprin beta subunit precursor consists of a signal peptide (aa 1 - 21), a pro region (aa 22 - 61), and a mature chain (aa 62 - 701) containing the following domains: catalytic (aa 62 - 259), MAM (aa 260 - 429), MATH (aa 430 - 585), EGF-like (aa 604 - 644), transmembrane (aa 653 - 673), and cytoplasmic (aa 674 - 701).

• References:

1. Bond, J.S. and R.J. Beynon (1995) Protein Sci. 4:1247.

2. Stocker, W. et al. (1995) Protein Sci. 4:823.

3. Bertenshaw, G.P. et al. (2001) J. Biol. Chem. 276:13248.

4. Ishmael, F.T. et al. (2005) J. Biol. Chem. 280:13895.

• Entrez Gene IDs:

  4225 (Human); 17288 (Mouse)

• Alternate Names:

  EC 3.4.24.63; endopeptidase-2; MEP1B; meprin A subunit beta; meprin A, beta; Meprin B; Meprin beta Subunit; N-benzoyl-L-tyrosyl-p-amino-benzoic acid hydrolase beta subunit; N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit beta; PABA peptide hydrolase; PPH beta