• Species Reactivity

  Human

• Specificity

  Detects human Dectin?1/CLEC7A in direct ELISAs. In directs ELISAs, less than 10% cross-reactivity with recombinant human (rh) DLEC and no cross-reactivity with recombinant mouse Dectin-1 or rhDectin-2 is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 259931

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human Dectin?1/CLEC7A    
  Thr66-Met201    
  Accession # NP_072092

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  2.5 μg/10    ⁶ cells

  Human whole blood monocytes

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• Blockade of Receptor-ligand Interaction

  In a functional ELISA, 0.1-0.4 μg/mL of this antibody will block 50% of the binding of 75 ng/mL of biotinylated Laminarin to immobilized Recombinant Human Dectin-1/CLEC7A (Catalog # ) coated at 250 ng/mL (100 μL/well). At 3 μg/mL, this antibody will block >90% of the binding.

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

• Immunocytochemistry

  8-25 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dectin-1/CLEC7A

Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 33 kDa type II transmembrane C-type lectin that participates in the innate immune response to fungal pathogens. Although Dectin-1 structurally resembles other CLEC molecules, it binds its ligands in a calcium-independent manner (1, 2). Mature human Dectin-1 consists of a short N-terminal ITAM-containing cytoplasmic tail, a transmembrane segment, and a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain (3, 4). Alternate splicing generates one major splice form that lacks the stalk region (3?5). This isoform is expressed on the surface of monocytes, macrophages, myeloid DC, neutrophils, eosinophils, B cells, and CD4⁺ T cells (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (5?7). Yeast beta -glucan is accessible to Dectin?1 only during the process of cell budding. Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8?10). In the membrane, Dectin-1 colocalizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11?13). Dectin-1 also interacts with tetraspanin CD37. This increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Dectin-1 knockout mice show increased susceptibility to pathogenic infection (15?16). The CRD of human Dectin-1 shares 77%, 60%, and 60% amino acid (aa) sequence identity with that of bovine, mouse and rat Dectin-1, respectively. It shares 29%?39% aa sequence identity with the CRD of other subgroup members, including CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL.

• References:

1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.

2. Brown, G.D. (2006) Nat. Rev. Immunol. 6:33.

3. Hernanz-Falcon, P. et al. (2001) Immunogenetics 53:288.

4. Yokota, K. et al. (2001) Gene 272:51.

5. Willment, J.A. et al. (2001) J. Biol. Chem. 276:43818.

6. Willment, J.A. et al. (2005) Eur. J. Immunol. 35:1539.

7. Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771.

8. Gantner, B.N. et al. (2005) EMBO J. 24:1277.

9. Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107.

10. Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467.

11. Brown, G.D. et al. (2003) J. Exp. Med. 197:1119.

12. Yadav, M. and J.S. Schorey (2006) Blood 108:3168.

13. Suram, S. et al. (2006) J. Biol. Chem. 281:5506.

14. Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154.

15. Saijo, S. et al. (2007) Nat. Immunol. 8:39.

16. Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.

• Entrez Gene IDs:

  64581 (Human); 56644 (Mouse)

• Alternate Names:

  Beta-glucan receptor; BGR; CD369; CLEC7A; CLECSF12; CLECSF12DC-associated C-type lectin 1; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 12; C-type lectin domain family 7 member A; C-type lectin domain family 7, member A; C-type lectin superfamily member 12; Dectin1; Dectin-1; DECTIN1CANDF4; Dendritic cell-associated C-type lectin 1; dendritic cell-associated C-type lectin-1; hDectin-1; lectin-like receptor 1