• Species Reactivity

  Mouse

• Specificity

  Detects mouse TSP50 in direct ELISAs and Western blots. In direct ELISAs, no cross?reactivity with recombinant human TSP50 is observed.

• Source

  Monoclonal Rat IgG    _2B Clone # 260514

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse TSP50    
  His58-Leu439    
  Accession # NP_666339.2

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 μg/mL

  Recombinant Mouse TSP50

• 
  

• Immunoprecipitation

  25 μg/mL

  Conditioned cell culture medium spiked with Recombinant Mouse TSP50,

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSP50

TSP50 (Testes-Specific Protease 50) may play a role in spermatogenesis and may also function as an oncogene.

• Long Name:

  Testes-specific Protease 50

• Entrez Gene IDs:

  29122 (Human); 235631 (Mouse)

• Alternate Names:

  Cancer/testis antigen 20; CT20probable threonine protease PRSS50; EC 3.4.25.-; protease, serine, 50; Serine protease 50; testes-specific protease 50; Testis-specific protease-like protein 50; TSP50; TSP50testes specific protease 50