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Human Snail Affinity Purified Alexa Fluor 488 Polyclonal Ab 25 TESTS

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產(chǎn)品介紹

    基本參數(shù)

    詳細說明

    • Species Reactivity

      Human

    • Specificity

      Detects human Snail in direct ELISAs.

    • Source

      Polyclonal Goat IgG

    • Purification

      Antigen Affinity-purified

    • Immunogen

      E. coli-derived recombinant human Snail    
      Pro2-Arg264    
      Accession # O95863

    • Formulation

      Supplied in a saline solution containing BSA and Sodium Azide.

    • Label

      Alexa Fluor 488

    Applications

    • Recommended    
      Concentration

      Sample

    • Intracellular Staining by Flow Cytometry

      5 μL/10    6 cells

      See below


    Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

    Data Examples

    Intracellular Staining by Flow Cytometry      
         

    Detection of Snail in A549 Human Cell Line by Flow Cytometry. A549 human lung carcinoma cell line was stained with Goat Anti-Human Snail Alexa Fluor? 488?conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC3639G, filled histogram) or isotype control antibody (Catalog # , open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # ). View our protocol for .

    Preparation and Storage

    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

    • Stability & Storage

      Protect from light.    Do not freeze.    

      • 12 months from date of receipt, 2 to 8 °C as supplied.

    Background: Snail

    Snail is predicted 29 kDa nuclear zinc finger transcriptional repressor that contains an N-terminal basic SNAG domain followed by three classical and one atypical zinc finger domains. During development, Snail is required for the establishment of left-right axis asymmetry. It also regulates the transcription of E-cadherin and other genes involved in epithelial-mesenchymal transitions during cancer progression. Human Snail shares 88% amino acid sequence identity with mouse and rat Snail.

    • Entrez Gene IDs:

      6615 (Human); 20613 (Mouse); 116490 (Rat)

    • Alternate Names:

      Protein sna; Protein snail homolog 1; SLUGH2; SNA; SNAH; SNAHdJ710H13.1; SNAI1; snail 1 (drosophila homolog), zinc finger protein; snail 1 homolog; snail 1 zinc finger protein; snail 1, zinc finger protein; snail homolog 1 (Drosophila); Snail; zinc finger protein SNAI1











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