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Human CCR7 Alexa Fluor 700 MAb (Clone 150503) 25 TESTS

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Human CCR7 Alexa Fluor 700 MAb (Clone 150503) 25 TESTS信息二維碼

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產(chǎn)品介紹

    基本參數(shù)

    詳細(xì)說明

    • Species Reactivity

      Human

    • Specificity

      Detects human CCR7.

    • Source

      Monoclonal Mouse IgG    2A Clone # 150503

    • Purification

      Protein A or G purified from hybridoma culture supernatant

    • Immunogen

      Human CCR7 transfectants    
      Met1-Pro378    
      Accession # AAA58615

    • Formulation

      Supplied in a saline solution containing BSA and Sodium Azide.

    • Label

      Alexa Fluor 700

    Applications

    • Recommended    
      Concentration

      Sample

    • Flow Cytometry

      5 μL/10    6 cells

      See below


    Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

    Data Examples

    Flow Cytometry      
         

    Detection of CCR7 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD4 PE?conjugated Monoclonal Antibody (Catalog # ) and either (A) Mouse Anti-Human CCR7 Alexa Fluor? 700?conjugated Monoclonal Antibody (Catalog # FAB197N) or (B) Mouse IgG2A Alexa Fluor 700 Isotype Control (Catalog # ). View our protocol for .

    Flow Cytometry      
         

    Detection of CCR7 in HEK293 Human Cell Line Transfected with Human CCR7 and eGFP by Flow Cytometry. The specificity of Mouse Anti-Human CCR7 Clone 150503 was demonstrated by its ability to react with (A) HEK293 human embryonic kidney cell line transfected with human CCR7 and not react with (B) irrelevant HEK293 transfectants. Antibody binding was monitored using Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ).

    Preparation and Storage

    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

    • Stability & Storage

      Protect from light.    Do not freeze.    

      • 12 months from date of receipt, 2 to 8 °C as supplied.

    Background: CCR7

    CCR7 is a 7 transmembrane G protein coupled chemokine receptor. CCR7 is expressed on T cells and mature dendritic cells and transduces chemotactic signals in response to CCL19 and CCL21. Human CCR7 shares 87% amino acid sequence identity with mouse CCR7.

    • Entrez Gene IDs:

      1236 (Human); 12775 (Mouse); 287673 (Rat)

    • Alternate Names:

      BLR2C-C chemokine receptor type 7; C-C CKR-7; CC-CKR-7; CCR7; CCR-7; CD197 antigen; CD197; CDw197CC chemokine receptor 7; chemokine (C-C motif) receptor 7; CMKBR7chemokine (C-C) receptor 7; EBI1EBV-induced G protein-coupled receptor 1; EBV-induced G-protein coupled receptor 1; Epstein-Barr virus induced gene 1; Epstein-Barr virus induced G-protein coupled receptor; Epstein-Barr virus-induced G-protein coupled receptor 1; EVI1; lymphocyte-specific G protein-coupled peptide receptor; MIP-3 beta receptor










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