• Species Reactivity

  Human

• Specificity

  Detects human FGF-16 in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant human (rh) FGF-3, -5, -8c, and -11 is observed and no cross-reactivity with rhFGF acidic, rhFGF basic, rhFGF-4, -6, -7, -8, -9, -10, -12, -13, -17, -18, or -19 is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 209511

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human FGF-16    
  Ala2-Arg207    
  Accession # O43320

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 μg/mL

  Recombinant Human FGF?16 (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-16

Fibroblast growth factor 16 (FGF-16) belongs to the large FGF family which has at least 23 members (1). All FGF family members are heparin-binding growth factors with a core 120 amino acid (aa) FGF domain that allows for a common tertiary structure. FGFs are expressed during embryonic development and in restricted adult tissues. They act on cells of mesodermal and neuroectodermal origin to regulate diverse physiologic functions including angiogenesis, cell growth, pattern formation, embryonic development, metabolic regulation, cell migration, neurotrophic effects and tissue repair (2, 3). Signaling receptors for FGFs are type I transmembrane receptor tyrosine kinases belonging to the immunoglobulin (Ig) superfamily. Four distinct but related classes of FGF receptors, FGF R1, 2, 3, and 4, exist. Through alternative splicing, multiple isoforms for FGF R1, 2 and 3, with distinct ligand recognition profiles, are also generated (3). Human FGF-16 cDNA predicts a 207 aa precursor protein with one N-linked glycosylation site. FGF-16 lacks a typical signal peptide but is efficiently generated by mechanisms other than the classical protein secretion pathway. Among FGF family members, FGF-16 is most similar to FGF-9, sharing 73% aa sequence homology. Human FGF-16 shares 99% and 98.6% aa sequence identity with the mouse and rat FGF-16, respectively. In rat embryos, FGF-16 message is expressed predominantly in brown adipocytes. In adult animals, it is localized primarily in heart tissue. FGF-16 binds to and activates FGF receptor 4 (4). FGF-16 induces proliferation of primary adipocytes and oligodendrocytes in vitro and stimulates liver weight increase in vivo (4, 5). The expression pattern of FGF-16 and its effect on adipocyte proliferation suggest a role for this protein on the proliferation of embryonic brown adipose tissue (4).

• References:

1. Miyake, A. et al. (1998) Biochem. Biophys. Res. Com. 243:148.

2. Goldfarb, M. (1996) Cytokine and Growth Factor Reviews 7:311.

3. Green, P. et al. (1996) BioEssays 18:639.

4. Konishi, M. et al. (2000) J. Biol. Chem. 275:12119.

5. Danilenko, D.M. et al. (2000) Archiv. Biochem. Biophys. 361:34.

• Long Name:

  Fibroblast Growth Factor 16

• Entrez Gene IDs:

  8823 (Human)

• Alternate Names:

  FGF16; FGF-16; fibroblast growth factor 16