• Species Reactivity

  Mouse

• Specificity

  Detects mouse PACAP/ADCYAP1 in direct ELISAs and Western blots. In direct ELISAs, approximately 12% cross?reactivity with recombinant human PACAP is observed.

• Source

  Polyclonal Sheep IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant mouse PACAP/ADCYAP1    
  Pro26-Leu175    
  Accession # O70176

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PACAP/ADCYAP1

PACAP (Pituitary adenylate cyclase activating polypeptide) is a 17 kDa (predicted) member of the VIP/secretin/glucagon/GHRH superfamily of peptides. It is expressed by anterior pituitary gonadotrophs, neurons in the CNS and PNS, spermatids, adrenal chromaffin cells, plus macrophages and lymphocytes. PACAP has multiple effects. On mast cells, it induces histamine release; on lymphocytes, PACAP promotes Th2 development; in bone, PACAP inhibits osteoclastogenesis; and in the pituitary, PACAP facilitates the release of LH (in females), GH and alpha ?MSH. Mouse PACAP proprecursor is 151 amino acids (aa) in length (aa 25?175). It undergoes proteolytic processing to generate two C?terminal amidated peptides termed PACAP-38 (aa 131?168) and PACAP-27 (aa 131?157). In general, PACAP-38 represents the dominant form. However, the ratio between the -38 and -27 forms differs in a cell-specific manner. Over aa 25?175, mouse PACAP shares 95% and 81% aa identity with rat and human PACAP, respectively.

• Long Name:

  Pituitary Adenylate Cyclase-activating Polypeptide

• Entrez Gene IDs:

  116 (Human); 11516 (Mouse); 24166 (Rat)

• Alternate Names:

  ADCYAP1; adenylate cyclase activating polypeptide 1 (pituitary); PACAP; PACAPMGC126852; pituitary adenylate cyclase-activating polypeptide