• Species Reactivity

  Human

• Specificity

  Detects human CXCL9/MIG in ELISAs and Western blots. In ELISAs, does not cross-react with recombinant mouse (rm) CXCL9, recombinant human CXCL10.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 49106

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human CXCL9/MIG    
  Thr23-Thr125    
  Accession # Q07325

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Fluorescein

Applications

• Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  10 μL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCL9/MIG

CXCL9, a member of the alpha  subfamily of chemokines that lack the ELR domain, was initially identified as a lymphokine-activated gene in mouse macrophages. Human CXCL9 was subsequently cloned using mouse MIG cDNA as a probe. The CXCL9 gene is induced in macrophages and in primary glial cells of the central nervous system specifically in response to IFN-gamma. CXCL9 has been shown to be a chemoattractant for activated T-lymphocytes and TIL but not for neutrophils or monocytes. The human CXCL9 cDNA encodes a 125 amino acid (aa) residue precursor protein with a 22 aa residue signal peptide that is cleaved to yield a 103 aa residue mature protein. CXCL9 has an extended carboxy-terminus containing greater than 50% basic aa residues and is larger than most other chemokines. The carboxy-terminal residues of CXCL9 are prone to proteolytic cleavage resulting in size heterogeneity of natural and recombinant CXCL9. CXCL9 with large carboxy-terminal deletions have been shown to have diminished activity in the calcium flux assay. A chemokine receptor (CXCR3) specific for CXCL9 and IP-10 has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes. The E. coli-expressed CXCL9 preparations produced at R&D Systems have been shown to contain greater than 80% full length CXCL9.

• References:

1. Loetscher, M. et al. (1996) J. Exp. Med. 184:963.

2. Liao, F. et al. (1995) J. Exp. Med. 182:1301.

3. Vanguri, P. (1995) J. Neuroimmunol. 56:35.

• Entrez Gene IDs:

  4283 (Human); 17329 (Mouse); 246759 (Rat)

• Alternate Names:

  chemokine (C-X-C motif) ligand 9; CMK; crg-10; C-X-C motif chemokine 9; CXCL9; Gamma-interferon-induced monokine; Humig; MIG; MIGSmall-inducible cytokine B9; monokine induced by gamma interferon; SCYB9Monokine induced by interferon-gamma