• Purity

  >97%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.01 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured in a cell proliferation assay using NR6R?3T3 mouse fibroblast cells. Rizzino, A.     et al. (1988) Cancer Res.     48:4266; Thomas, K.     et al. (1987) Methods Enzymol.     147:120. The ED    ₅₀ for this effect is 0.1-0.3 ng/mL in the presence of 10 μg/mL of heparin.

• Source

  E. coli-derived Phe16-Asp155, with an N-terminal Met

• Accession #

• N-terminal Sequence    
  Analysis

  Met

• Predicted Molecular Mass

  15.5 kDa

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: FGF acidic

FGF acidic, also known as FGF-1, ECGF, and HBGF-1, is a 17 kDa nonglycosylated member of the FGF family of mitogenic peptides. FGF acidic, which is produced by multiple cell types, stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis (1 - 3). Human FGF acidic shares 54% amino acid sequence identity with FGF basic and 17%?33% with other human FGFs. It shares 92%, 96%, 96%, and 96% aa sequence identity with bovine, mouse, porcine, and rat FGF acidic, respectively, and exhibits considerable species crossreactivity. Alternate splicing generates a truncated isoform of human FGF acidic that consists of the N-terminal 40% of the molecule and functions as a receptor antagonist (4). During its nonclassical secretion, FGF acidic associates with S100A13, copper ions, and the C2A domain of synaptotagmin 1 (5). It is released extracellularly as a disulfide-linked homodimer and is stored in complex with extracellular heparan sulfate (6). The ability of heparan sulfate to bind FGF acidic is determined by its pattern of sulfation, and alterations in this pattern during embryogenesis thereby regulate FGF acidic bioactivity (7). The association of FGF acidic with heparan sulfate is a prerequisite for its subsequent interaction with FGF receptors (8, 9). Ligation triggers receptor dimerization, transphosphorylation, and internalization of receptor/FGF complexes (10). Internalized FGF acidic can translocate to the cytosol with the assistance of Hsp90 and then migrate to the nucleus by means of its two nuclear localization signals (11-13). The phosphorylation of FGF acidic by nuclear PKC delta triggers its active export to the cytosol where it is dephosphorylated and degraded (14, 15). Intracellular FGF acidic functions as a survival factor by inhibiting p53 activity and proapoptotic signaling (16).

• References:

1. Jaye, M. et al. (1986) Science 233:541.

2. Galzie, Z. et al. (1997) Biochem. Cell Biol. 75:669.

3. Presta, M. et al. (2005) Cytokine Growth Factor Rev. 16:159.

4. Yu, Y.L. et al. (1992) J. Exp. Med. 175:1073.

5. Rajalingam, D. et al. (2007) Biochemistry 46:9225.

6. Guerrini, M. et al. (2007) Curr. Pharm. Des. 13:2045.

7. Allen, B.L. and A.C. Rapraeger (2003) J. Cell Biol. 163:637.

8. Robinson, C.J. et al. (2005) J. Biol. Chem. 280:42274.

9. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.

10. Wiedlocha, A. and V. Sorensen (2004) Curr. Top. Microbiol. Immunol. 286:45.

11. Wesche, J. et al. (2006) J. Biol. Chem. 281:11405.

12. Imamura, T. et al. (1990) Science 249:1567.

13. Wesche, J. et al. (2005) Biochemistry 44:6071.

14. Wiedlocha, A. et al. (2005) Mol. Biol. Cell 16:794.

15. Nilsen, T. et al. (2007) J. Biol. Chem. 282:26245.

16. Bouleau, S. et al. (2005) Oncogene 24:7839.

• Long Name:

  Fibroblast Growth Factor acidic

• Entrez Gene IDs:

  2246 (Human); 14164 (Mouse); 25317 (Rat); 281160 (Bovine)

• Alternate Names:

  AFGF; alpha; alpha-ECGF; beta-ECGF; ECGF; ECGFB; ECGF-betaAcidic fibroblast growth factor; endothelial cell growth factor, beta; FGF acidic; FGF1; FGF-1; FGFABeta-endothelial cell growth factor; FGF-alpha; fibroblast growth factor 1 (acidic); GLIO703; HBGF1; HBGF-1; heparin-binding growth factor 1