• Purity

  >85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie? Blue stain.

• Activity

  Reaction conditions will need to be optimized for each specific application. We recommend an initial recombinant human USP15 concentration of 5-20 nM when using Ubiquitin-AMC or Ubiquitin-Rh110 substrates (U-550, U-555).

• Source

  Spodoptera frugiperda,     Sf 21 (baculovirus)-derived Contains an C-terminal 6-His tag

• Accession #

• Predicted Molecular Mass

  113 kDa

Background: USP15

Ubiquitin carboxyl-terminal hydrolase 15 (USP15) is a specialized cysteine protease with a predicted molecular weight of 112 kDa.  USP15 is a member of the peptidase C19 family and the human protein shares 98% amino acid sequence identity with its mouse ortholog.  USP15 is a regulator of the TGF beta signaling pathway, where it may promote signaling strength by deubiquitination of SMAD proteins and/or the TGF beta R1.  Other reported targets of USP15 include histone H2B, NF kappa BI alpha, MDM2 and human papillomavirus type 16 E6 protein.

• References:

   

   

1. Eichhorn P.J.,           et al. (2012)           Nat. Med.           18: 429-435

2. Long L.,           et al. (2014)           J. Biol. Chem.           289: 8916-8930

3. Voss R.M.,           et al. (2009)           J. Virol.           83: 8885-8892

4. Zou Q., et al. (2014) Nat Immunol. 15: 562-70

• Long Name:

  Ubiquitin Specific Protease 15

• Entrez Gene IDs:

  9958 (Human); 14479 (Mouse); 171329 (Rat)

• Alternate Names:

  Deubiquitinating enzyme 15; EC 3.1.2.15; EC 3.4.19.12; Gcap18; KIAA0529; KIAA0529ubiquitin carboxyl-terminal hydrolase 15; MGC131982; MGC149838; MGC74854; ubiquitin specific peptidase 15; ubiquitin specific protease 15; ubiquitin thioesterase 15; Ubiquitin thiolesterase 15; Ubiquitin-specific-processing protease 15; Ubp109; UNPH-2; UNPH4; USP15