• Assay Type

  Magnetic bead-based multiplex assay for the Luminex    ^? MAGPIX    ^? platform

• Format

  96-well microplate and antibody-coated magnetic beads*

• Analytes Detected

  Please see table below.

• Performance Validation

  For standard curves, intra-assay and inter-assay precision, recovery, and linearity data for the different analyte-specific beads, please see product datasheets for corresponding individual Bead Sets (below).

* Antibody-coated beads supplied in compatible Bead Sets, sold separately (see below).

Product Features

• Simultaneously detect multiple analytes

• Magnetic format allows for easier wash steps

• User selects specific analytes of interest

• Requires small sample size

• Undergoes similar validation testing as Quantikine^? ELISA Kits

• Precise and Specific

Base Kit Contents

• Standard Cocktail(s)

• Bead Diluent

• Biotin Antibody Diluent

• Calibrator Diluent(s)

• Streptavidin-PE

• Wash Buffer Concentrate

• Mixing Bottles

• Round-bottom Microplate

• Foil Plate Sealers

Bead Set Contents

• Antibody-coated magnetic beads

• Biotinylated detection antibody

    Information for Compatiable Analytes

For performance data for the different analyte-specific beads, please see product datasheets for the compatible individual Bead Sets.

Validated Samples and Recommended Starting Dilution

More Information about Magnetic Bead-Based Multiple Kits for the Luminex Platform

Magnetic  multiplex kits are designed for use with the  CCD Imager. Alternatively, kits can be used with the  or Bio-Rad^? Bio-Plex^?, dual laser, flow-based sorting and detection platforms.

Analyte-specific antibodies are pre-coated onto color-coded magnetic microparticles. Microparticles, standards and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, streptavidin-phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated antibody, is added to each well. A final wash removes unbound Streptavidin-PE, the microparticles are resuspended in buffer and read using the Luminex MAGPIX Analyzer. A magnet in the analyzer captures and holds the superparamagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a Bio-Rad Bio-Plex dual laser, flow-based systems.

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