• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 10    ⁴ cells/well are added to rhICAM-2/Fc Chimera coated plates (12.5 μg/mL with 100 μL/well), >70% will adhere after 1 hour incubation at 37 °C.    
     Optimal dilutions should be determined by each laboratory for each application.  

• Source

  Mouse myeloma cell line, NS0-derived

  Human ICAM-2 (Lys25 - Gln223) Accession # CAA33630	IEGRMD	Human IgG1 (Pro100 - Lys330)	6-His tag
  N-terminus			C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Lys25

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  50 kDa (monomer)

• SDS-PAGE

  86 kDa, reducing conditions

Background: ICAM-2/CD102

Intercellular Adhesion Molecule-2 (ICAM-2, CD102), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). ICAM-2 is constitutively expressed at high levels on vascular endothelial cells and lymphohematopoietic cells. ICAM-2 mediated adhesion has been shown to provide a co-stimulatory signal for T cell aggregation, NK cytotoxicity and NK cell migration.

• References:

1. Somersalo, K. et al. (1995) J. Biol. Chem. 270:8629.

2. Hayflick, J. et al. (1998) Immunologic Res. 17:313.

• Long Name:

  Intercellular Adhesion Molecule 2

• Entrez Gene IDs:

  3384 (Human); 15896 (Mouse)

• Alternate Names:

  CD102 antigen; CD102; ICAM2; ICAM-2; intercellular adhesion molecule 2