• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie? Blue stain

• Activity

  Recombinant Human His6-Ufc1 is a member of the UFM1-conjugating (E2) enzyme family that receives UFM1 from a UFM1-activating (E1) enzyme and subsequently interacts with a UFM1 ligase (E3) to conjugate UFM1 to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-Ufc1 concentration of 0.1-1 μM.

• Source

  E. coli-derived Contains an N-terminal 6-His tag

• Accession #

• Predicted Molecular Mass

  22 kDa

Background: Ufc1

Ubiquitin-fold Modifier Conjugating Enzyme 1 (Ufc1) has been identified as an E2-like conjugating enzyme for Ubiquitin Fold Modifier 1 (Ufm1) (1). Ufc1 is a 116 amino acid (aa) protein with a predicted molecular weight of 19 kDa. Human Ufc1 exhibits 97% and 98% aa identity to its mouse and rat orthologs, respectively. The process of attaching Ufm1 to target proteins is termed ufmylation. It is thought to occur in a manner analogous to the ubiquitin system, including stepwise enzymatic reactions involving Ubiquitin-activating (E1)-, Ubiquiting-conjugating (E2)-, and Ubiquitin Ligase (E3)-like enzymes. In addition to Ufc1, other members of the Ufm1-conjugation system include the E1-like enzyme Uba5, E3-like enzyme Ufl1, and deconjugating enzymes UfSP1 and UfSP2 (1-3). This protein contains an N-terminal His6 tag.

• References:

1. Komatsu, J. et al. (2004) EMBO J. 23:1977.

2. Tatsumi, K. et al. (2010) 285:5417.

3. Kang, S.H. et al. (2007) J. Biol. Chem. 282:5256.

• Long Name:

  Ubiquitin-fold Modifier Conjugating Enzyme 1

• Entrez Gene IDs:

  51506 (Human); 66155 (Mouse); 445268 (Rat)

• Alternate Names:

  HSPC155; ubiquitin-fold modifier conjugating enzyme 1; ubiquitin-fold modifier-conjugating enzyme 1; Ufc1; Ufm1-conjugating enzyme 1