• Species Reactivity

  Mouse

• Specificity

  Detects mouse Integrin alpha 5/CD49e in direct ELISAs and Western blots.

• Source

  Monoclonal Rat IgG2b Clone # 235112

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha 5/CD49e    
  Phe45-Asn999 (Gln68Glu)    
  Accession # P11688

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 350

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 μg/10    ⁶ cells

  B16?F1 mouse melanoma cell line

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Integrin alpha 5/CD49e

Integrin alpha 5 subunit, also called CD49e, associates with the Integrin beta 1 subunit (CD29) to form the VLA-5 complex. It is expressed on thymocytes, mast cells, activated T lymphocytes, and some mouse cell lines. It functions as a receptor for fibronectin (1?4).

• References:

1. Springer, T.A.         et al. (1982) Immunol. Rev.         68:171.

2. Kinashi, T. and T.A. Springer (1994) Blood Cells         20:25.

3. Halvorson, M.J.         et al. (1995) J. Immunol.         55:4567.

4. Uhlenkott, E.C. et al. (1996) Clin. Exp. Metastasis 14:125.

• Entrez Gene IDs:

  3678 (Human); 16402 (Mouse)

• Alternate Names:

  CD49 antigen-like family member E; CD49e antigen; CD49e; Fibronectin receptor subunit alpha; fibronectin receptor, alpha subunit; FNRAVLA5A; Integrin alpha 5; integrin alpha-5; Integrin alpha-F; integrin, alpha 5 (fibronectin receptor, alpha polypeptide); ITGA5; very late activation protein 5, alpha subunit; VLA-5 alpha; VLA-5